摘要
目的利用CO_(2)点阵激光精准控制烧伤强度及面积,并摸索最适能量强度,制备皮肤浅Ⅱ度至深Ⅱ度烧伤模型。方法首先通过50 mJ、80 mJ能量强度的CO_(2)点阵激光对小鼠进行造模,得到两组不同能量强度的烧伤模型;观察造模后烧伤部位表面皮肤变化情况,在造模后0、3、7、14 d拍照,观察伤口愈合情况,分析伤口愈合率;随后,对不同时间点所取烧伤部位组织进行HE染色、Masson染色,以及ColⅠ、ColⅢ免疫组织化学染色,并进行结果分析。结果对不同时间点的组织进行组织学染色发现,两组的激光打孔深度、毛囊再生情况及瘢痕生成情况存在显著差异;ColⅠ、ColⅢ免疫组织化学染色结果显示,两组间胶原蛋白再生情况同样存在显著差异。综合分析发现,80 mJ能量强度对小鼠皮肤组织所造成的损伤更符合深Ⅱ度烧伤模型特点。结论本研究成功利用CO_(2)点阵激光在小鼠背部构建出皮肤组织再生明显障碍的背侧深Ⅱ度烧伤模型,并通过组织学、免疫组织化学等方法对其损伤程度进行了详细评估,最终筛选出80 mJ的能量强度用于构建皮肤深Ⅱ度烧伤模型,有望为烧伤病理生理研究及皮肤组织再生药物筛选提供新工具。
Objective To accurately control the intensity and area of burns by using fractional carbon dioxide laser,and to explore the optimal energy intensity to prepare a model of deep second-degree skin burns.Methods Firstly,two groups of burn models with different energy intensities were obtained by using 50 mJ and 80 mJ CO_(2)fractional lasers,and the skin changes on the surface of the burn site were observed after burn modeling,and photographs were taken at 0,3,7 and 14 days after modeling to observe the healing of burns and analyze the wound healing rate.Subsequently,HE staining,Masson staining,ColⅠand ColⅢimmunohistochemical staining were performed on the burn tissues taken at different time points,and the results were analyzed.Results Histological staining of the materials at different time points showed that there were significant differences in the depth of laser perforation,hair follicle regeneration and scar formation between the two groups.The immunohistochemical staining results of ColⅠand ColⅢshowed that there were also significant differences in collagen regeneration between the two groups.Comprehensive analysis showed that the damage caused by 80 mJ energy intensity to the skin tissue of mice was more consistent with the characteristics of the deep second-degree burn model.Conclusion In this study,a model of deep second-degree burns with obvious obstacles in skin tissue regeneration was successfully constructed on the back of mice by carbon dioxide fractional laser,and the degree of damage was evaluated in detail by histology,immunohistochemistry,etc.,and finally the energy intensity of 80 mJ was screened for the construction of a deep seconddegree burn model,which is expected to provide a new and favorable tool for studying the pathophysiology of burns and the screening of drugs for skin tissue regeneration.
作者
王湘莹
卢婷婷
李凯祥
曾俊文
王会景
王伟
邓丹
付炜
Xiangying;LU Tingting;LI Kaixiang;ZENG Junwen;WANG Huijing;WANG Wei;DENG Dan;FU Wei(Instituteof Pediatric Translational Medicine,Shanghai Children's Medical Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China;Department of Pediatric Cardiothoracic SurgeryShanghai Children's Medical Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China;Department of Dermatology,Shanghai Children's Medical Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China;Shanghai Key Laboratory of Tissue Engineering,Shanghai 200011,China.)
出处
《组织工程与重建外科》
CAS
2024年第1期51-57,共7页
Journal of Tissue Engineering and Reconstructive Surgery
基金
上海交通大学“交大之星”计划医工交叉研究基金(YG2021GD04)。
关键词
深Ⅱ度烧伤
二氧化碳点阵激光
皮肤损伤
Deep second-degree burn model
Carbon dioxide fractional laser
Skin injury
