摘要
【背景】多杀性巴氏杆菌可导致猪肺疫、牛出血性败血症和兔出血性败血症等多种疾病,严重威胁多种动物畜牧养殖业的健康发展。【目的】重庆某兔场送检一批病死兔,为研究其病原和治疗方法,对病原进行了微生物分离和全基因组测序分析。【方法】从2022年重庆某兔场送检兔病料中进行细菌分离纯化、生化试验、16S rRNA基因鉴定、荚膜血清型分型、药敏试验和毒力基因检测,同时通过全基因组测序结果进行毒力、耐药基因注释和遗传进化等分子生物学信息分析。【结果】该菌为兔源A:ST74多杀性巴氏杆菌,命名为LXSS001,基因组序列上传到NCBI数据库(登录号为CP119523.1),药敏试验显示该菌对四环素、杆菌肽、复方新诺明和磺胺异恶唑耐药,对头孢噻肟、头孢哌酮和丁胺卡那等药物敏感。全基因组长度为2480671 bp,并注释到了58个毒力基因和9类药物的靶向抗药基因。通过联合建树表明其与3480株一致性最高。【结论】本研究完成了一株A型多杀性巴氏杆菌的分离鉴定和全基因组测序,并揭示了其与国内外其他分离株的进化关系,为多杀性巴氏杆菌的后续研究提供了参考依据。
[Background]Pasteurella multocida can cause various diseases such as porcine pulmonary disease,bovine hemorrhagic sepsis,and rabbit hemorrhagic sepsis,posing a serious threat to the healthy development of the animal husbandry in China.[Objective]A rabbit farm in Chongqing sent a batch of sick and dead rabbits for examination.To study their pathogens and treatment methods,we carried out microbial isolation and whole genome sequencing for the pathogens.[Methods]The bacterial isolation and purification,biochemical testing,16S rRNA gene identification,capsule serotyping,drug susceptibility testing,and virulence gene testing were conducted with the diseased rabbit samples from a rabbit farm in Chongqing,2022.The whole genome sequencing results were employed to annotate the virulence genes and drug resistance genes and build the phylogenetic tree.[Results]The isolate was identified as a rabbit-derived type A Pasteurella multocida strain belonged to the sequence type ST74 and named LXSS001.The genome sequence was uploaded to NCBI(collection number:CP119523.1).The strain was resistant to tetracycline,compound sulfamethoxazole,and sulfamethoxazole,while it was sensitive to cefotaxime,cefoperazone,and amikacin and so on.The strain had the whole genome length of 2480671 bp and carried 58 virulence genes and resistance genes for 9 classes of antibiotics.The phylogenetic tree showed that LXSS001 had the highest homology with the 3480 strain.[Conclusion]This study completed the isolation,identification,and whole genome sequencing of a type A P.multocida strain and revealed its phylogenetic relationship with other isolates,providing a basis for the subsequent research on P.multocida.
作者
罗逊
丁碧荷
王印
罗燕
姚学萍
任梅渗
杨泽晓
LUO Xun;DING Bihe;WANG Yin;LUO Yan;YAO Xueping;REN Meishen;YANG Zexiao(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,Sichuan,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2024年第2期582-598,共17页
Microbiology China
基金
四川省科技计划(2023ZHYZ0006)。
关键词
兔
多杀性巴氏杆菌
全基因组测序
致病性
耐药性
rabbit
Pasteurella multocida
whole genome sequencing
pathogenicity
drug resistance
