摘要
目的探究血清趋化因子(C-X-C基序)配体3(CXCL3)在结直肠癌早期诊断和预后评估中的价值。方法生物信息学分析鉴定CXCL3在结直肠癌中的诊断效能。采用酶联免疫吸附法(ELISA)定量检测200例结直肠癌患者和200名健康人群血清CXCL3浓度。同时,构建测试队列、验证队列和交叉队列来确定趋化因子CXCL3的诊断潜力。单因素和多因素Cox回归分析影响结直肠癌预后的独立危险因素,确定趋化因子CXCL3作为结直肠癌预后指标的可行性,组间差异性分析采用χ2检验。结果生物信息学分析显示CXCL3基因在结直肠癌组织中的表达显著高于正常组织。结直肠癌患者血清CXCL3表达水平显著升高(P<0.01)。CXCL3在测试队列、验证队列及其各自的交叉队列中的曲线下面积(AUC)分别为0.806、0.850、0.834和0.826(P<0.01)。当CXCL3与相关肿瘤抗原CEA、CA125、CA19-9联合诊断时,其AUC高于把CXCL3作为单一诊断指标(0.881比0.829,P<0.01)。肿瘤大小的亚组分析表明CXCL3在肿瘤大小<4 cm时最有效(AUC=0.810,P<0.01)。高CXCL3表达组的总生存期(OS)缩短(P<0.01),血清CXCL3水平(P=0.005)是结直肠患者预后的独立影响因素。结论血清CXCL3对结直肠癌有较好的诊断价值。CXCL3高表达组结直肠癌患者中位生存期较短,提示其预后不良。
Objective To explore the diagnostic and prognostic value of chemokine(C-X-C motif)ligand 3(CXCL3)in colorectal cancer(CRC).Methods Bioinformatics analysis was done to identify the diagnostic potency of CXCL3 in CRC.The serum levels of chemokine CXCL3 in 200 CRC patients and 200 healthy people were measured quantitatively by enzyme linked immunosorbent assay(ELISA).At the same time,the diagnostic potential of chemokine CXCL3 was determined by constructing test queue,validation queue and cross queue.The independent risk factors affecting the prognosis of CRC were analyzed by univariate and multivariate Cox regression to determine whether the chemokine CXCL3 can be used as a prognostic indicator of CRC.Comparison between groups was performed by Chi-Squared test.Results The expression level of serum CXCL3 in CRC patients was significantly increased(P<0.01).The area under the curve(AUC)of CXCL3 in the test queue,verification queue and their respective cross queues were 0.806,0.850,0.834 and 0.826 respectively(P<0.01),indicating that CXCL3 had good diagnostic efficiency,proving its value in the diagnosis of CRC.When CXCL3 was diagnosed with CEA,CA125 and CA19-9,its AUC was higher than CXCL3 as a single diagnostic index(0.881 vs.0.829,P<0.01),which improved the diagnostic efficiency.Subgroup analysis of tumor size showed that CXCL3 was most effective when the tumor was<4 cm(AUC=0.810,P<0.01).Overall survival(OS)of the high CXCL3 expression group was shortened(P<0.01),and serum CXCL3 level(P<0.01)was an independent predictor for prognosis of CRC patients.Conclusion Serum CXCL3 has a good diagnostic value for CRC.The median OS in the CXCL3 high expression group was shortened,predicting poor prognosis,which means the expression level of CXCL3 is expected to become a non-invasive evaluation indicator for accurate prognosis assessment of CRC patients.
作者
陈书宁
田雨
秦艳
刘彦魁
周心一
韩伟峰
齐晓薇
Chen Shuning;Tian Yu;Qin Yan;Liu yankui;Zhou Xinyi;Han Weifeng;Qi Xiaowei(Department of Pathology,the Affiliated Hospital of Jiangnan University,Wuxi 214122,China)
出处
《中华实验外科杂志》
CAS
2024年第1期137-140,共4页
Chinese Journal of Experimental Surgery
基金
无锡市医学创新团队(CXTDBY2021003)
无锡市转化医学研究所项目(LCYJ202309)。
