摘要
对黑紫色叶紫薇品种‘赤红’叶片进行高通量测序,并利用Trinity(2.8.6)对测序数据进行从头组装,共获得23791条unigene,其中91.86%的序列在NR、GO、COG、KEGG等5大数据库中得到注释;NR数据库比对到的unigene数量最多,占比91.93%,随后依次为SWISS(67.95%)、COG(60.43%)、GO(51.37%)和KEGG(35.43%)。在NR数据库中,‘赤红’紫薇所有的unigene仅比对到1个物种——石榴(Punica granatum L.),表明同属于千屈菜科的紫薇和石榴亲缘关系较近。从转录组中共筛选到4775个SSR位点,包括6种核苷酸重复类型,其中A/T和AG/CT类型占比最高。对色素合成相关通路基因进行分析,共挖掘到18个与类黄酮和花青素相关的基因,其中花青素合成通路中的UGT79B1、BZ1、UGT75C1可能是‘赤红’紫薇形成紫黑色叶的关键基因。
The high-throughput transcriptome sequencing for the leaves of Lagerstroemia indica'Ebony Fire'yielded 23791 unigenes after de novo assembly by Trinity(2.8.6).Among them,91.86%of the unigenes were annotated in NR,SWISS,COG,GO,and KEGG.The NR database annotated the largest number of unigenes,which accounted for 91.93%,followed by SWISS(67.95%),COG(60.43%),GO(51.37%),and KEGG(35.43%).Only one species of Punica granatum L.was compared in the NR database,indicating the high sequence homology between L.indica L.and P.granatum L..A total of 4775 SSR loci were screened out from the transcriptome,involving six nucleotide repeat types,of which A/T and AG/CT accounted for the largest proportion.The mining of genes involved in pigment synthesis revealed 18 genes associated with flavonoids and anthocyanin.UGT79B1,BZ1,and UGT75C1 in the anthocyanin synthesis pathway may be the key genes for L.indica'Ebony Fire'to produce purple black leaves.
作者
刘玉军
秦波
李媚
黄耀恒
刘芳
黄欣
刘莉
LIU Yu-jun;QIN Bo;LI Mei;HUANG Yao-heng;LIU Fang;HUANG Xin;LIU Li(Nanning Arboretum,Nanning 530031,PRC;Guangxi Forestry Research Institute,Guangxi Key Laboratory of Special Non-Wood Forest Cultivation and Utilization,Nanning 530002,PRC)
出处
《湖南农业科学》
2024年第1期1-6,共6页
Hunan Agricultural Sciences
基金
广西自筹经费林业科技项目(桂林科研〔2022ZC〕第73号)。
关键词
紫薇品种‘赤红’
转录组
基因注释
色素合成关键基因
Lagerstroemia indica'Ebony Fire'
transcriptome
gene annotation
key genes in pigment synthesis
