摘要
目的分析长链非编码RNA(lncRNA)SNHG25在前列腺癌中的表达及其意义,挖掘前列腺癌诊断和预后的生物标志物和潜在的治疗靶点。方法基于TCGA数据库对目标基因SNHG25进行差异分析、生存分析和临床相关性分析;在UALCAN数据库中分析SNHG25在前列腺癌中的表达与临床及病理特征的关系;对SNHG25进行lncRNA-miRNA-mRNA相关性分析,在前列腺癌中构建相关ceRNA调控网络;将前列腺癌样本分为SNHG25的高低表达两组,筛选出与SNHG25相关的差异基因后进行富集分析。结果SNHG25在前列腺癌样本中的表达相较于正常前列腺样本中的表达明显上调(P<0.001),SNHG25低表达组患者的无进展生存期明显长于高表达组患者(P<0.001);在前列腺癌患者SNHG25高、低表达两组之间,年龄、T分期、N分期均无明显差异,SNHG25的表达水平与前列腺癌患者的Gleason评分无明显相关性(P>0.05)。对SNHG25进行lncRNA-miRNA-mRNA相关性分析构建出SNHG25/miR-330-3p/DLX1、RPL22L1调控网络。结论SNHG25在前列腺癌组织中高表达,与前列腺癌患者的不良预后相关;SNHG25的表达水平与前列腺癌患者年龄、T分期、N分期和Gleason评分无明显相关性;SNHG25/miR-330-3p/DLX1、RPL22L1调控网络在前列腺癌的发生发展中可能发挥重要作用,SNHG25可能成为前列腺癌诊断和预后的生物标志物和潜在的治疗靶点。
Objective To analyze the expression of lncRNA SNHG25 in prostate cancer and its significance,so as to explore the biomarkers and potential therapeutic targets for the diagnosis and prognosis of this disease.Methods Based on the TCGA database,differential,survival,and clinical correlation analyses of SNHG25 were performed.SNHG25 expression in prostate cancer was analyzed in the UALCAN database to determine its relationship with the clinical and pathological characteristics.The lncRNA-miRNA-mRNA correlation analysis was performed.The relevant ceRNA regulatory network was constructed.Prostate cancer samples were divided into high and low SNHG25 expression groups,and differential SNHG25 related genes were filtered and then enriched.Results SNHG25 expression was significantly upregulated in prostate cancer specimens compared to normal prostate specimens(P<0.001),and the progression-free survival of the low SNHG25 expression group was significantly longer than that of the high SNHG25 expression group(P<0.001).There were no significant differences in age,T-stage and N-stage between the two groups,and there was no significant correlation between the expression of SNHG25 and Gleason score(P>0.05).Regulatory networks of SNHG25/miR-330-3p/DLX1 and RPL22L1 were constructed.Conclusion SNHG25 is highly expressed in prostate cancer tissues and correlated with poor prognosis.SNHG25 expression does not significantly correlate with age,T-stage,N-stage,and Gleason score.SNHG25/miR-330-3p/DLX1 and RPL22L1 regulatory networks may play an important role in the development of prostate cancer.SNHG25 may become a biomarker and potential therapeutic target for prostate cancer.
作者
康海
刘晓颖
赵洁
周松
蒋湘勇
李铁求
KANG Hai;LIU Xiaoying;ZHAO Jie;ZHOU Song;JIANG Xiangyong;LI Tieqiu(Department of Urology,The First Affiliated Hospital of Hunan Normal University/Hunan Provincial People's Hospital,Changsha 410002,China;Department of Pathology,The First Affiliated Hospital of Hunan Normal University/Hunan Provincial People's Hospital,Changsha 410002,China)
出处
《现代泌尿外科杂志》
CAS
2024年第3期224-231,共8页
Journal of Modern Urology
基金
长沙市自然科学基金项目(No.kq2208112)
湖南省人民医院仁术重点项目(No.RS2022A13)
湖南省卫健委科研项目(No.202204052798)
湖南省卫生健康高层次人才支持计划资助项目(No.湘卫函[2023]78号)
湖南省中医药科研计划项目(No.2021041)。
