摘要
【目的】为建立大鼻吻鮈(Rhinogobio nasutus)规模化人工繁育技术,并为其生产性育苗工艺和种质资源保护等提供科学依据。【方法】采用野生的大鼻吻鮈经人工催产获得受精卵,记录并分析其胚胎及仔鱼发育各时期的形态特征。【结果】大鼻吻鮈成熟卵呈圆球形,浅绿色,为漂流性卵,卵径(0.60±0.08)mm,受精卵吸水充分膨胀后卵径(0.76±0.08)mm。在水温(23.2±1.0)℃,pH 8.71~8.86条件下,受精卵3.67 h发育至囊胚期,5.95 h发育至原肠期,9.55 h胚体形成,13.38 h器官开始形成,19.98 h出现肌肉效应情况,24.05 h心跳出现,40.43 h完成出膜,至仔鱼期。大鼻吻鮈初孵仔鱼全长3.00~4.00 mm,心跳101~132次/min;在水温26℃条件下,仔鱼2 d全部吸收完卵黄,部分泄殖孔已开,3 d全部开口摄食。【结论】利用人工催产的方法记录、分析和描述了大鼻吻鮈的胚胎发育过程,并且成功获得了大鼻吻鮈初孵仔鱼,其胚胎发育过程经历胚盘期、卵裂期、囊胚期、原肠胚期、神经胚期、器官形成期及孵化出膜7个阶段,每个发育阶段又有若干个发育时期。
【Objective】The aim of this study was to establish large-scale artificial breeding technology and provide a scientific basis for the establishment of productive seedling and the protection of germplasm resources for Rhinogobio nasutus.【Method】Fertilized eggs of Rhinogobio nasutus were obtained through artificially-induced spawning,and the morphological characteristics of embryo and larval development were recorded and analyzed at a constant water temperature of(23.2±1.0)°C.【Result】The unfertilized mature eggs were spherical,light green,and drifting,with a diameter of(0.60±0.08)mm.After fertilization and hydration,the fully swollen eggs became translucent,with a diameter of(0.76±0.08)mm.Under the conditions of water temperature(23.2±1.0)°C and pH 8.71~8.86,the fertilized eggs developed into the blastula stage in 3.67 hours,the gastrula stage in 5.95 hours,embryo body formation in 9.55 hours,organ formation in 13.38 hours,muscle effect in 19.98 hours,and heartbeat in 24.05 hours.After 40.43 hours,embryo development was completed,and the larval stage was reached.The overall length of newly hatched larvae ranged from about 3.00 to 4.00 mm,with a heartbeat of 101~132 beats per minute.The yolk of the larvae was completely absorbed within 2 days,and in some larvae,the cloaca was opened.All larvae started ingestion 3 days after hatching,under the condition of a water temperature of 26℃.【Conclusion】This study recorded,analyzed,and described the process of embryonic development of the big-nosed minnow using artificial labor induction.Hatching larvae of Rhinogobio nasutus were successfully obtained.The embryonic development process went through seven stages,including the embryo dish stage,cleavage stage,blastocyst stage,gastrula stage,neuroembryonic stage,organ formation stage,and hatching out of the membrane.Each development stage consisted of several phases.
作者
杨瑞兰
刘彦斌
赵红雪
刘凯
杨立强
肖伟
赛清云
田永华
赖章龙
阮超岭
柳婷
刘哲
连总强
YANG Ruian;LIU Yanbin;ZHAO Hongxue;LIU Kai;YANG Liqiang;XIAO Wei;SAI Qingyun;TIAN Yonghua;LAI Zhangong;RUAN Chaoing;LIU Ting;LIU Zhe;LIAN Zongqiang(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Ningxia Fisheries Research Institute,Yinchuan 750001,China;College of Life Science,Ningxia University,Yinchuan 750021,China;Ningxia Fishery Engineering Technology Research Center,Yinchuan 750001,China;College of Life and Geographic Sciences,Kashi University,Kashi 844000,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2024年第1期49-58,共10页
Journal of Gansu Agricultural University
基金
宁夏重点研发计划项目“大鼻吻鮈种质资源保护遗传学及生殖发育和人工繁育技术研究”(2023BCF01013)
农业农村部财政专项“黄河渔业资源与环境调查”项目(HHDC-2022-02)
2022年自治区重点研发计划黄河流域生态环境保护与高质量发展科技支撑专项“黄河宁夏段公路斜拉桥绿色低碳建管养关键技术研究”(2022BEG02056)。
关键词
大鼻吻鮈
胚胎发育
仔鱼
形态特征
Rhinogobio nasutus
embryonic development
larval
morphological characteristics
