摘要
【目的】研究叶用芥菜主要农艺性状的配合力,为加快叶用芥菜新选不育系应用及强优势杂交组合选育提供理论依据。【方法】以4个叶用芥菜不育系稳定株系美宽A、台宽A、大芥A和明长A为母本,3个宽柄芥自交系V0331、V0332和V0333为父本,按不完全双列杂交配制12个杂交组合,对其11个农艺性状进行配合力方差分析,并估算基因型方差贡献率和遗传率。【结果】芥菜杂交组合间存在真实的遗传差异,不育系和自交系11个主要农艺性状的一般配合力方差均达显著(P<0.05)或极显著(P<0.01,下同)水平,除株幅外其他性状特殊配合力方差均达极显著水平。通过对配合力相对效应值的分析,筛选出不育系台宽A、明长A和自交系V0331为优秀亲本;推选出3个优势的组合美宽A×V0333、明长A×V0333和台宽A×V0331。父本均为宽柄芥类型的情况下,芥菜杂交组合的株高、株幅、叶长、叶柄长、叶柄宽、中肋宽和叶片数7个性状的表型主要取决于芥菜不育系的遗传;叶宽、叶柄厚、中肋厚和单株产量4个性状则主要受父本自交系的影响。11个主要农艺性状的广义遗传率均达70.00%以上;叶柄长、中肋宽、叶长、叶片数、叶柄宽、单株产量、中肋厚、株幅和叶柄厚9个性状的狭义遗传率均大于55.00%,加性遗传效应大,育种上可在低世代进行选择;株高和叶宽的狭义遗传率分别为27.41%和35.74%,受环境和双亲互作影响大,育种上适宜在高世代选择。【结论】台宽A和明长A是较理想的不育系亲本,可扩大组合配制加以利用;组合美宽A×V0333、明长A×V0333和台宽A×V0331为优势组合,具有良好的利用价值,建议进一步进行区域试验。
【Objective】The combining ability of main agronomic traits of leaf mustard was studied in order to provide theoretical basis for accelerating the application of newly selected sterile lines of leaf mustard and the breeding of strong dominant hybrid combination.【Method】Four sterile lines Meikuan A,Taikuan A,Dajie A and Mingchang A were as fe‐male parents and three inbred lines V0331,V0332 and V0333 were as male parents,incomplete diallel cross design was used to group twelve hybrid combinations.The variance analysis of combining ability for 11 agronomic traits was carried out,and the contribution rate of genotype variance and heritability were estimated.【Result】There were real genetic differen-ces among the cross combinations of leaf mustard,the variances of general combining ability of 11 main agronomic traits of sterile lines and inbred lines reached significant(P<0.05)or extremely significant(P<0.01,the same below)levels.The variance of special combining ability(SCA)of hybrid combinations was extremely significant except plant width.Through the analysis of the relative effect of combining ability,the male sterile line Taikuan A,Mingchanga A and inbred line V0331 were selected as excellent parents.The combination of three advantages,Meikuan A×V0333,Mingchang A×V0333 and Taiwan A×V0331,was selected.In the case of the male parent was broad-stem mustard type,the phenotypes of plant height,plant width,leaf length,petiole length,petiole width,middle rib width and leaf number of leaf mustard hybrids were mainly determined by the inheritance of mustard male sterile lines.Leaf width,petiole thickness,middle rib thickness and yield per plant were mainly affected by male parent inbred lines.The general heritability of 11 main agro‐nomic traits was more than 70.00%.The narrow sense heritability of 9 traits,namely,petiole length,middle rib width,leaf length,mid-leaf number,petiole width,yield per plant,middle rib thickness,plant width and petiole thickness,was more than 55.00%,the additive genetic effect was large,and the breeding could be selected in the lower generation.The narrow sense heritability of plant height and leaf width were 27.41%and 35.74%,respectively.The two traits were greatly influenced by environment and the interaction between parents,breeding was suitable for high generation selection.【Con‐clusion】Taikuan A and Mingchang A are ideal parents of sterile lines,which can be used by expanding the combination.Meikuan A×V0333,Mingchang A×V0333 and Taikuan A×V0331 are the dominant combinations,they have utilization values,it is suggested that further regional test should be carried out.
作者
张双照
ZHANG Shuang-zhao(Institute of Agricultural Sciences,Longyan,Fujian 364000,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2023年第12期3650-3656,共7页
Journal of Southern Agriculture
基金
国家现代农业产业技术体系建设项目(CARS-24-G-07)
福建省科技计划项目(2021N0054)
福建省省属公益类科研院所基本科研业务专项(2020R1031007)
福建省农业科学院农业科技专项(ZZZYCXZX202301)。
关键词
叶用芥菜
不育系
农艺性状
配合力分析
leaf mustard
sterile line
agronomic traits
combining ability analysis
