摘要
通过研究液体培养时不同氮含量(0.4%、0.6%、0.8%、1.0%、1.2%、1.4%)对菌丝生长、碳氮代谢相关酶活性及基因表达的影响,筛选大球盖菇(Stropharia rugosoannulata)菌丝生长最适氮源。结果表明:豆粕粉是大球盖菇菌丝生长最适氮源。氮含量为0.6%时,菌丝生长速度最快。在实验范围内,对照(氮含量为0)的亚硝酸盐还原酶活性较高,1.2%氮含量的天冬酰胺合成酶活性较高,0.6%氮含量的谷氨酸合酶活性最高;0.6%氮含量的内切β-1,4-葡聚糖酶活性最高,β-1,3-葡聚糖酶活性较高;0.6%氮含量的谷氨酸合酶基因表达量最高,1.2%氮含量的谷氨酸脱氢酶基因表达量较高;对照的β-1,3-葡聚糖酶基因和半纤维素酶基因表达量均最高。研究结果可为大球盖菇液体菌种开发和应用提供参考。
To study the optimal nitrogen source for mycelial growth of Stropharia rugosoannulata,it was cultured under different nitrogen sources(corncob,wheat bran,rice bran,rapeseed cake,bean pulp and calces)and nitrogen contents(0.4%,0.6%,0.8%,1.0%,1.2%and 1.4%),and then measured for mycelial growth,enzyme activities and gene expression pertaining to nitrogen and carbon metabolism.The results showed that bean pulp powder was the optimal nitrogen source for mycelial growth.The mycelial growth rate of S.rugosoannulata was the greatest when the nitrogen content was 0.6%.Within the experimental range,the activity of nitrite reductase(NiR)in the control(0 nitrogen content)and the asparagine synthase(AS)activity of 1.2%nitrogen content were high.Both the activity and gene expression of glutamine-2-oxoglutarate aminotransferase(GOGAT)were the highest under 0.6%nitrogen content.For cellulose enzymes,the activity of endogenousβ-1,4-glucanase(Cx)was the highest under 0.6%nitrogen content,and the activity of exogenousβ-1,4-glucanase(C1)was also high under 0.6%nitrogen content.The glutamate dehydrogenase gene was highly expressed under 1.2%nitrogen content.On the other hand,the expression levels ofβ-1,3-glucanase gene and hemicellulase(ACX)gene in the control were the highest.This study provided a reference for the development and application of liquid spawn of S.rugosoannulata.
作者
张津京
卓馨怡
冀浩
郝海波
岳一鸿
王倩
肖婷婷
陈辉
ZHANG Jinjing;ZHUO Xinyi;JI Hao;HAO Haibo;YUE Yihong;WANG Qian;XIAO Tingting;CHEN Hui(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences;Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;College of Life Sciences,Nanjing Agricultural University,Nanjing 210014,Jiangsu,China)
出处
《食用菌学报》
CSCD
北大核心
2024年第2期10-18,共9页
Acta Edulis Fungi
基金
上海市现代农业产业技术体系[沪农科产字(2023)第9号]
国家自然科学基金(31971571)。
关键词
大球盖菇
氮源
纤维素酶
基因表达
Stropharia rugosoannulata
nitrogen resource
cellulase
gene expression
