摘要
目的对金银花Lonicera japonica TCP(LjTCP)基因家族进行鉴定分析,以期为进一步研究LjTCPs的功能机制奠定基础。方法以金银花基因组数据为基础,通过生物信息学系统分析TCP基因家族在染色体上的分布、系统进化、基因结构、启动子元件及其表达模式。结果共鉴定出17个LjTCPs,其中16个LjTCPs分布在9条染色体上,LjTCP17未定位到染色体上,基于系统进化树和多重序列比对,LjTCP基因家族可以分为PCF、CIN和CYC/TB13个亚家族,分别包含9、6、2个LjTCPs。基因组内共线性分析表明,全基因组复制和片段性复制在LjTCP基因家族进化中发挥了重要作用。LjTCP基因家族启动子包含许多与植物生长发育、激素响应,以及非生物和生物胁迫相关的调控元件。表达模式分析表明,LjTCP基因在金银花花发育初期表达量较高,随着发育进程表达量呈现下降趋势,并且LjTCP05、LjTCP13、LjTCP14、LjTCP16和LjTCP17基因在花青素含量不同的品种中表达量存在差异。对6个具有低温响应元件的LjTCPs在冷胁迫下的基因表达研究表明,大部分基因在冷处理后基因表达呈现上升趋势。结论金银花TCP基因家族包含17个成员,各成员的分子特征和表达模式存在差异。
Objective Genome-wide identification of the TCP gene family in Lonicera japonica lays the foundation for further studying the functional mechanisms of the LjTCPs.Methods Based on the genomic data of L.japonica,the distribution on chromosomes,phylogenetic evolution,gene structure,promoter elements,and expression patterns of the TCP gene family were analyzed using bioinformatics tools.Results A total of 17 LjTCPs were identified,with 16 LjTCPs distributed on nine chromosomes and LjTCP17 not located on any chromosome.Based on the phylogenetic tree and multiple sequence alignment,the LjTCP gene family could be divided into three subfamilies:PCF,CIN and CYC/TB1,which contained nine,six,and two LjTCPs,respectively.The genomic collinearity analysis indicated that whole genome duplication and segmental duplication played important roles in the evolution of the LjTCP gene family.The promoter regions of the LjTCP gene family contained many regulatory elements related to plant growth and development,hormone responses,as well as biotic and abiotic stress.Expression pattern analysis showed that LjTCP genes had higher expression levels in the early stage of flower development,which decreased during the development process.Furthermore,the expression levels of LjTCP05,LjTCP13,LjTCP14,LjTCP16 and LjTCP17 genes varied among varieties with different anthocyanin contents.Expression profiles studies of six LjTCPs with low-temperature responsive elements under cold stress showed that most genes exhibited an up-regulation in gene expression after cold treatment.Conclusion The L.japonica TCP gene family consists of 17 members,and each member displayed distinct molecular characteristics and expression patterns.
作者
谭政委
郭水柱
苏小雨
孙瑶
余永亮
李磊
张利超
许兰杰
鲁丹丹
安素妨
李春明
梁慧珍
王子君
TAN Zhengwei;GUO Shuizhu;SU Xiaoyu;SUN Yao;YU Yongliang;LI Lei;ZHANG Lichao;XU Lanjie;LU Dandan;AN Sufang;LI Chunming;LIANG Huizhen;WANG Zijun(Institute of Chinese Herbel Medicines,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Zhongjing Wanxi Pharmaceutical Co.,Ltd.,Nanyang 474550,China;Technology Service Center on Ecological Planting of Chinese Herbal Medicine in Chengde,Chengde 067000,China;Seed Management Department of Zhumadian,Zhumadian 463000,China)
出处
《中草药》
CAS
CSCD
北大核心
2024年第5期1665-1676,共12页
Chinese Traditional and Herbal Drugs
基金
河南省重大科技专项(221100310400)
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-21)
河南省科技攻关项目(242102112173,242102112161,232102110198,232102110243,232102110262)
中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”(2060302)
河南省重点研发计划(231111110800)。
关键词
金银花
TCP转录因子
基因家族
共线性分析
系统进化
表达模式
Lonicera japonica Thunb.
TCP transcription factor
gene family
collinearity analysis
phylogenetic evolution
expression profiles
