摘要
本研究以柳叶马鞭草盆栽苗为实验材料,探索其快繁体系。结果表明,将5~6 cm的带腋芽茎段以75%酒精与20%NaClO组合设计试验,发现用75%酒精消毒30 s, 20%NaClO消毒8 min的效果最好,7 d后污染率为12.78%,死亡率为4.44%,成活率达82.78%。将3~4 cm腋芽放在添加不同浓度6-BA、NAA的MS培养基中进行增殖诱导培养,发现在培养基为MS+1.5 mg/L 6-BA+0.10 mg/L NAA培养时,30 d后诱导率为100%,增殖系数达4.77,产生的丛生芽数目多。将3~4 cm的丛生芽剪成单芽,在培养基为MS+0.10 mg/L NAA进行培养时,15 d后株高差为4.64 cm,芽苗健壮且无枯萎现象。将长势好、高度5 cm左右的柳叶马鞭草无根苗置于培养基中探索IBA、NAA激素浓度,在MS+0.10 mg/L IBA+0.05 mg/L NAA条件下,15 d后生根率为100%,平均生根数为40.43,平均根长为3.27 cm;45 d后,平均根长为14.33 cm。进一步以蛭石代替琼脂作为基质,15 d后生根率为100%,平均根长可达4.50 cm, 45 d后平均根长可达42.33 cm,显著高于琼脂基质中的平均根长。自然光条件下炼苗4 d后,移栽至配比为V_(蛭石)∶V_(营养土)=1∶2的基质上,30 d后成活率为93.33%。
This study used potted seedlings of Verbena bonariensis as experimental materials to explore its rapid propagation system.The results showed that a 5-6 cm stem segment with axillary buds was designed with a combination of 75%alcohol and 20%NaClO.It was found that the best effect was achieved under the conditions of 75%alcohol disinfection for 30 seconds and 20%NaClO disinfection for 8 minutes.After 7 days,the pollution rate was 12.78%,the mortality rate was 4.44%,and the survival rate was 82.78%.The 3-4 cm buds were cultured in MS medium with different concentrations of 6-BA and NAA for proliferation induction.It was found that when the medium was MS+1.5 mg/L 6-BA+0.10 mg/L NAA,the induction rate was 100%after 30 days,and the proliferation coefficient reached 4.77,resulting in a large number of clustered buds.3-4 cm clusters of buds were cut into single buds and culture them on MS+0.10 mg/L NAA medium.After 15 days,the height difference of the plant was 4.64 cm,and the seedlings were robust and without withering.The rootless seedlings of Verbena bonariensis with good growth and a height of about 5 cm were placed in a culture medium to explore the concentration of IBA and NAA hormones.Under the conditions of MS+0.10 mg/L IBA+0.05 mg/L NAA,the rooting rate was 100%after 15 days,with an average rooting number of 40.43 and an average root length of 3.27 cm.After 45 days,the average root length was 14.33 cm.Further replacing agar with vermiculite as the substrate,the rooting rate was 100%after 15 days,and the average root length could reach 4.50 cm.After 45 days,the average root length could reach 42.33 cm,significantly greater than the average root length in the agar substrate.When the root length and number of Verbena bonariensis plants in the root culture medium were suitable,sterile seedlings with similar growth were selected for subsequent seedling cultivation and transplantation.It was found that after 4 days of seedling cultivation under natural light conditions,they were transplanted onto a substrate of V_(vermiculite)∶V_(nutrient soil)=1∶2,and after 30 days,the survival rate was 93.33%.
作者
王思思
蔡菊
张涛
袁丹丹
杨秀柳
李岩
WANG Sisi;CAI Ju;ZHANG Tao;YUAN Dandan;YANG Xiuliu;LI Yan(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China)
出处
《种子》
北大核心
2024年第2期135-141,149,F0003,共9页
Seed
基金
国家自然科学基金项目(32160722)
贵州省基础研究计划重点项目(黔科合基础-ZK[2023]重点006)
中央引导地方科技发展资金(黔科合中引地[2023]009)。
关键词
柳叶马鞭草
离体快繁
增殖
Verbena bonariensis
propagation in vitro
proliferation
