摘要
瓜蚜是黄瓜上重要害虫,严重影响黄瓜产量和品质,筛选和利用抗蚜品种是减轻黄瓜蚜虫为害的有效手段。以瓜蚜不同抗性的13个黄瓜品种为材料,研究黄瓜叶片可溶性蛋白质、氨基酸及可溶性糖3种营养物质含量与抗蚜性关系,以建立快速鉴定黄瓜品种抗蚜性的方法。结果表明:黄瓜叶片可溶性糖、游离脯氨酸含量与瓜蚜数量呈显著正相关,其回归方程分别为Y=0.0002X+0.0371(r=0.6195,P=0.0021)和Y=0.5620X+34.0324(r=0.7423,P=0.0251);叶片可溶性蛋白质含量与叶片瓜蚜种群数量无显著相关性。对可溶性糖(X_(1))和游离脯氨酸(X_(2))进行逐步回归分析,其回归方程为Y=-69.6650+1294.7471X_(1)+0.8678X_(2)(R^(2)=0.8036^(*))。黄瓜营养物质中可溶性糖和游离脯氨酸对瓜蚜的抗性具有重要作用,可作为鉴定黄瓜抗蚜性的指标。
Aphid,as an important pest in cucumber production,seriously affects the yield and quality of cucumber.Screening and utilization of resistant aphid varieties is an effective method to reduce the damage of aphid in cucumber.In this study,we explored the relationship between the inclusions(carbohydrate,soluble proteins and amino acid)in leaves from 13 cucumber cultivars and aphid numbers,in order to establish an efficient method to identify the cucumber aphid-resistance varieties.The results showed thar there were significant positive correlation between the contents of soluble sugar and free prolines in cucumber leaves and the number of aphid.The regression equations were Y=0.0002X+0.0371(r=0.6195,P=0.0021),and Y=0.5620X+34.0324(r=0.7423,P=0.0251),respectively.The content of soluble protein in leaves of cucumber was not affected by the number of melon aphid.Stepwise regression analysis was performed for soluble sugar(X_(1))and free prolines(X_(2));thus,the regression equation and path coefficient were explained as follows:Y=-69.6650+1294.7471X_(1)+0.8678X_(2)(R^(2)=0.8036^(*)).These results suggested that the soluble sugar and free prolines in cucumber play important roles in aphid resistance and thus can be used as a standard to identify the resistance of cucumber to aphid.
作者
夏彭飞
任佳
韩杜斌
许学文
陈申
周福才
陈学好
XIA Pengfei;REN Jia;HAN Dubin;XU Xuewen;CHEN Shen;ZHOU Fucai;CHEN Xuehao(Nanjing Xinchuang Vegetable Molecular Breeding Research Institute Company Limited,Nanjing 211899.China;College of Plant Protection,Yangzhou University,Yangzhou 225009,China;College of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou 225009,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2024年第1期133-138,共6页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
江苏省农业科技自主创新资金项目[CX(20)3165]
镇江市1+1+N新型农业技术推广项目[ZJNJ(2019)05]。
关键词
黄瓜
瓜蚜
抗虫性
抗生性
cucumber
melon aphid
resistance
antibiosis
