摘要
通过盆栽试验,研究铁锰改性生物炭(BC_(FM))对水稻Cd吸收和土壤微生物群落结构的影响.结果表明,BC_(FM)可有效降低水稻对Cd的吸收,改变土壤微生物群落结构.与对照相比,添加0.5~2g/kg BC_(FM)后土壤有机质、土壤阳离子交换量、土壤总Fe和总Mn无显著变化;而添加4g/kg BC_(FM)后,土壤有机质、土壤阳离子交换量、土壤总Fe和Mn含量分别显著(P<0.05)提高了13.6%、13.58%、5.0%和12.1%,同时土壤中有效态Cd含量显著(P<0.05)降低67.9%,且水稻茎、叶和糙米中Cd含量分别显著(P<0.05)降低74.3%、44.9%和84.9%.添加BC_(FM)后,水稻根际土壤Firmicutes、Proteobacteria等门水平物种的相对丰度提高,而Bacteroidota、Patescibacteria、Desulfobacterota和Nitrospirae等门水平物种的相对丰度降低.热图分析结果表明,BC_(FM)可提高水稻根际土壤中Bacillus、Citrifermentans、Geobacter、Desulfovibrio等参与铁锰氧化还原和硫酸盐还原细菌的相对丰度.RDA和网络分析表明,BC_(FM)可通过影响土壤总Fe、有机质含量和Cd的生物有效性来改变土壤微生物群落结构,促进土壤细菌间的正交互作用.因此,BC_(FM)可作为一种有效的、生态安全的钝化剂用于Cd污染农田土壤修复.
In this study,the effects of Fe-Mn modified biochar(BC_(FM))on rice Cd uptake and soil microbial community structure were further studied through pot experiments.The results showed that BC_(FM) could effectively reduce the Cd uptake in rice and change the soil microbial community structure.As compared with the control,the content of soil organic matter,soil cation exchange capacity,soil total Fe,and total Mn were slightly changed with the application of 0.5~2g/kg BC_(FM).However,the content of soil organic matter,cation exchange capacity,total Fe and Mn were increased significantly(P<0.05)by 13.6%,13.58%,5.0%,and 12.1%,respectively,with the application of 4g/kg BC_(FM).Meanwhile,the content of available Cd in soil decreased significantly(P<0.05)by 67.9%,and the content of Cd in stems,leaves,and brown rice decreased significantly(P<0.05)by 74.3%,44.9%,and 84.9%,respectively.The relative abundance of Firmicutes and Proteobacteria at the phylum level in rice rhizosphere soil was increased with the application of BC_(FM),while the relative abundance of Bacteroidota,Patescibacteria,Desulfobacterota,and Nitrospirae was decreased.Heatmap analysis showed that BC_(FM) could promote the relative abundance of Bacillus,Citrifermentans,Geobacter,and Desulfovibrio,which were involved in iron-manganese redox and sulfate reduction in rice rhizosphere soil.Redundancy analysis and network analysis showed that BC_(FM) could promote positive interaction of soil microorganisms by affecting soil total Fe,organic matter,and available Cd content.Therefore,BC_(FM) could be used as an effective and ecological safe passivator for the remediation of Cd-contaminated paddy soil.
作者
王德正
谭文韬
曾鹏
马兰心
周航
辜娇峰
廖柏寒
WANG De-zheng;TAN Wen-tao;ZENGPeng;MA Lan-xin;ZHOU Hang;GU Jiao-feng;LIAO Bo-han(College of Environment Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2024年第4期2297-2308,共12页
China Environmental Science
基金
国家自然科学基金青年基金资助项目(42207008)
国家重点研发计划项目(2022YFD1700104-1)。
