摘要
目的制备柚皮素仿生纳米颗粒(MSN-Nar@PLTM),并考察其理化性质以及对心肌缺血再灌注损伤的保护作用。方法采用梯度离心反复冻融的方法提取血小板膜,通过浸渍法和超声法制备纳米粒。表征纳米粒的载药量、包封率、粒径、电位、形态和生物安全性等,验证血小板膜表面蛋白质的完整性,及该纳米材料对心肌缺血再灌注损伤的治疗效果。结果MSN-NAR@PLTM粒径为139.57±0.96 nm,Zeta电位-21.80±0.15 mV,载药量15.3%,包封率30.7%,具有完整的膜结构,良好的生物安全性,对损伤的心肌细胞具有保护作用。结论MSN-Nar@PLTM的仿生递送系统能够高效装载柚皮素,改善柚皮素在水中溶解性差的问题,提高其生物利用度,对心肌缺血再灌注损伤具有保护作用。
OBJECTIVE To prepare naringenin loaded platelt-mimetic nanoparticles(MSN-Nar@PLTM)and to investigate their physicochemical properties as well as their protective effects against myocardial ischemia-reperfusion injury.METHODS Platelet membranes were extracted by gradient centrifugation with repeated freezing and thawing,and nanoparticles were prepared by impregnation method and sonication.The drug loading,encapsulation rate,particle size,potential,morphology,and biosafety of the nanoparticles were also characterized to verify the integrity of the proteins on the surface of platelet membranes and the therapeutic effect of the nanomaterials on myocardial ischemia-reperfusion injury.RESULTS MSN-Nar@PLTM was 139.57±0.96 nm in diameter.The zeta potential was-21.80±0.15 mV,the loading capacity was 15.3%,and the encapsulation rate was 30.7%.MSN-Nar@PLTM had an intact membrane structure,good biosafety,and demonstrated a protective effect on damaged cardiomyocytes.CONCLUSION MSN-Nar@PLTM platelet-mimetic delivery system,which can load naringenin efficiently,addresses the issues of poor solubility and slow dissolution of naringenin after oral administration,improves its bioavailability,and has protective effects against myocardial ischemia-reperfusion injury.
作者
黄静
廖丽萍
肖雯婧
马婕
于波涛
HUANG Jing;LIAO Liping;XIAO Wenjing;MA Jie;YU Botao(School of Life and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610031 P.R.China;School of Pharmacy,North Sichuan Medical University,Nanchong,Sichuan,637000 P.R.China;Department of Pharmacy,the General Hospital of Western Theater Command of PLA,Chengdu,Sichuan,610083 P.R.China)
出处
《华西药学杂志》
CAS
CSCD
北大核心
2024年第2期146-150,共5页
West China Journal of Pharmaceutical Sciences
基金
中央直属高校专题研究项目(2682022ZTPY038)。
关键词
柚皮素
血小板膜
血小板仿生纳米粒
介孔二氧化硅
心肌缺血再灌注损伤
Naringenin
Platelet membrane
Platelet mimetic nanoparticles
Mesoporous silica
Myocardial ischemia-reperfusion injury
