摘要
为深入了解植物乳杆菌(Lactobacillus plantarum)YP36细菌素的抑菌机理,该研究利用高效液相色谱-串联质谱(HPLC-MS/MS)联用非标记定量蛋白质组学技术对细菌素处理前后植物乳杆菌SL32-2差异表达蛋白进行鉴定,并对差异蛋白进行代谢功能分析。结果表明,细菌素处理前后植物乳杆菌SL32-2发酵液中鉴定到的蛋白分别为1999个、2005个,显著上调和下调的蛋白质分别有15个和59个,另外有26个蛋白消失,33个蛋白诱导表达。所鉴定蛋白质中酶占比最大,其次为转运蛋白。基因本体论(GO)富集分析表明,显著性差异表达蛋白的功能条目共99条,主要集中在肽聚糖分解代谢和细胞壁大分子分解代谢、碳水化合物代谢以及嘧啶核苷酸代谢和生物合成等方面。京都基因与基因组百科全书(KEGG)富集分析表明,差异蛋白共涉及淀粉和蔗糖代谢途径、丙氨酸、天冬氨酸和谷氨酸代谢途径、辅因子生物合成途径和嘧啶代谢途径等53条通路。
In order to understand the antibacterial mechanism of bacteriocin from Lactobacillus plantarum YP36,the differential expression proteins of L.plantarum SL32-2 before and after bacteriocin treatment were analyzed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)combined with label-free quantitative proteomics technique,and the metabolic function of the differential proteins were analyzed.The results showed that 1999 and 2005 proteins were identified in the fermentation broth of L.plantarum SL32-2 before and after bacteriocin treatment respectively,15 and 59 proteins were significantly up-regulated and down-regulated,26 proteins were disappeared,and 33 proteins were induced to expression.Among the identified proteins,enzymes accounted for the largest proportion,followed by transporters.Gene Ontology(GO)enrichment analysis showed that there were 99 functional items of significantly differentially expressed proteins,which mainly focused on peptidoglycan catabolism,cell wall macromolecular catabolism,carbohydrate metabolism,pyrimidine nucleotide metabolism and biosynthesis.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that the differential proteins involved 53 pathways including starch and sucrose metabolism,alanine,aspartate and glutamate metabolism,cofactor biosynthesis and pyrimidine metabolism.
作者
王婷
来欢欢
赵微
崔美林
张秀红
WANG Ting;LAI Huanhuan;ZHAO Wei;CUI Meilin;ZHANG Xiuhong(School of Food Science,Shanxi Normal University,Taiyuan 030032,China;School of Life Science,Shanxi Normal University,Taiyuan 030032,China;Shanxi Engineering Research Center of Microbial Application Technologies,Taiyuan 030032,China)
出处
《中国酿造》
CAS
北大核心
2024年第4期98-104,共7页
China Brewing
基金
山西省自然科学基金项目(202103021223255,202203021221135)。
关键词
细菌素
高效液相色谱-串联质谱
蛋白质组学
抑菌机制
代谢功能分析
bacteriocin
high performance liquid chromatography tandem mass spectrometry
proteomics
bacteriostatic mechanism
metabolic function analysis
