摘要
为了探究四季秋海棠BsRbohD基因的功能,本研究以异源表达四季秋海棠BsRbohD基因的‘哥伦比亚’野生型拟南芥为材料,通过最大光化学效率(F_(v)/F_(m))、活性氧(ROS)水平、花色素苷含量及相关合成基因表达的变化来分析BsRbohD基因与花色素苷之间的关系。结果显示:四季秋海棠BsRbohD基因异源转入拟南芥后可正常表达。在对照条件下,两个过表达株系OE1、OE2的超氧阴离子(O_(2)·^(-))产生速率和过氧化氢(H_(2)O_(2))含量虽然略高于野生型,但F_(v)/F_(m)、花色苷含量和相关结构基因没有显著差异;高光条件下,与野生型相比,BsRbohD在OE1和OE2中显著表达,使得O_(2)·^(-)产生速率和H_(2)O_(2)含量显著增加;之后,花色素苷的含量及合成相关基因的表达响应高光而显著上调的时间显著提前、上调幅度也较野生型高。本研究的结果表明,四季秋海棠BsRbohD基因在拟南芥中异源过表达后,产生了较高的ROS水平,之后调控花色素苷合成相关基因的表达,最终生成花色素苷,为进一步明晰BsRbohD基因的功能提供理论支持。
In order to explore the function of the BsRbohD gene of Begonia semperflorens,the'Columbia'wild-type A rabidopsis thaliana heterologously expressing the BsRbohD gene of B.semperflorens was used as the material,analyzing the relationship between BsRbohD gene and anthocyanin through changing in maximum photochemical efficiency(F_(v)/F_(m)),reactive oxygen species(ROS)levels,anthocyanin content and related synthetic gene expression.The results showed that the BsRbohD gene of B.semperflorens was expressed normally after being heterologously transferred into Athaliana.Under control conditions,two transgenic lines(OE1 and OE2)did not exhibit significant differences in F_(v)/F_(m),anthocyanin content and anthocyanin-biosynthesis-related genes expression,although slight higher content of drogen peroxide(H_(2)O_(2))and production rate superoxide anion(0_(2)·^(-))were detected in OE1 and OE2.Under high light conditions,OE1 and OE2 showed higher expression level in BsRbohD,which consequently increased reactive oxygen species(ROS)level in OE1 and OE2 compared to WT;moreover,anthocyanin-biosynthesis-related genes showed quicker and more upregulation in OE1 and OE2,which consequently accumulated more anthocyanins than WT under high light conditions.These findings suggested that ectopic overexpression of BsRbohD in A.thaliana produced more ROS which triggered anthocyanin biosynthesis through upregulating anthocyanin-biosynthesis-related genes expression in OE1 and OE2.These findings provided theoretical support for further clarifying the function of BsRbohD gene.
作者
王瑞博
刘静
姚珂心
王阳
陈敏
刘胜男
李永华
张开明
Wang Ruibo;Liu Jing;Yao Kexin;Wang Yang;Chen Min;Liu Shengnan;Li Yonghua;Zhang Kaiming(College of Landscape Architecture and Art,Henan Agricultural University,Zhengzhou,450002)
出处
《分子植物育种》
CAS
北大核心
2024年第9期2822-2828,共7页
Molecular Plant Breeding
基金
河南省重点研发与推广专项(212102110013)
河南农业大学科技创新基金项目自然科学类青年创新基金(KJCX2018A04)共同资助。
