摘要
目的分析使用免疫检查点抑制剂(ICI)治疗后出现免疫相关不良反应(irAE)的非小细胞肺癌(NSCLC)患者的外周血T淋巴细胞亚群水平及其与血清细胞因子的关系。方法前瞻性纳入2022年4~10月于某院就诊的非小细胞肺癌患者47例,其中根据有无发生irAE分为irAE组(n=12)和对照组(n=35),采用流式细胞术检测患者外周血淋巴细胞亚群的比例,比较两组间淋巴细胞亚群比例的差异性,Pearson相关性分析细胞因子与淋巴细胞亚群水平的相关性。结果与对照组相比,irAE组患者外周血TCR-αβ^(+)PD1^(+)CD4^(-)CD^(8-)T细胞水平升高,差异具有统计学意义(P=0.045),其他T淋巴细胞亚群无明显差异。Pearson相关性分析结果显示外周血TCR-αβ^(+)PD1^(+)CD4^(-)CD8^(-)T细胞与血清干扰素-γ(IFN-γ)呈正相关关系(r=0.448;P=0.019)。结论irAE患者外周血TCR-αβ^(+)PD1^(+)CD4^(-)CD8^(-)T细胞水平升高,并且与血清IFN-γ水平正相关,有可能参与irAE的发生。
OBJECTIVE To analyze T lymphocyte subsets in peripheral blood of patients with non-small cell lung cancer(NSCLC)who developed immune-related adverse events(irAE)after treatment with immune checkpoint inhibitor(ICI).METHODS A total of 47 patients with NSCLC admitted to the First Affiliated Hospital of Guangzhou Medical University from April to October 2022 were included.Patients were divided into irAE group(n=12)and control group(n=35).Flow cytometry was used to detect the proportion of lymphocyte subsets in peripheral blood,and the difference in the proportion of lymphocyte subsets between two groups was compared.The correlation between cytokines and lymphocyte subsets was analyzed by Pearson correlation.RESULTS Compared with the control group,peripheral TCR-αβ^(+)PD1^(+)CD4^(-)CD8^(-)T cell level increased in the irAE group,and the difference was statistically significant(P=0.045).There was no significant difference in other T lymphocyte subsets.Pearson correlation analysis showed that TCR-αβ^(+)PD1^(+)CD4^(-)CD8^(-)TTcells in peripheral blood were positively correlated with serum interferon-γ(IFN-γ)(r=0.448,P=0.019).CONCLUSION The level of TCR-αβ^(+)PD1^(+)CD4^(-)CD8^(-)T cell increased in the peripheral blood of irAE patients and was positively correlated with serum IFN-γ level,which may be involved in the occurrence of irAE.
作者
陈婉文
蔡俊滢
何玉文
曾军
魏理
CHEN Wanwen;CAI Junying;HE Yuwen;ZENG Jun;WEI Li(Department of Pharmacy,The First Afiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 510120,China;Department of Pharmacy,Guangzhou Medical University,Guangzhou,Guangdong 511436,China;Department of Basic Medical Sciences,Guangzhou Medical University,Guangzhou,Guangdong 511436,China)
出处
《今日药学》
CAS
2024年第3期209-213,共5页
Pharmacy Today
基金
广东省医院药学研究基金(澳美基金,2023A30)
广州市科技计划项目(202102010186、202201020466)
广州医科大学学科建设项目药学拔尖人才培养项目(02-445-2301186)。
