摘要
目的 比较不同基原枳实水煎液黄酮类成分和抗氧化活性差异,为枳实的质量评价和临床用药提供参考。方法 建立HPLC法同时测定枳实水煎液中8种黄酮类成分(芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、柚皮素、橙皮素、川陈皮素、桔皮素)的含量,结合多元统计分析化学成分差异;采用DPPH法和ABTS法测定不同基原枳实水煎液的抗氧化活性,以灰色关联度分析和双变量相关性分析评价枳实水煎液中8种成分含量与抗氧化活性的相关性。结果 含量测定及统计分析结果表明不同基原枳实水煎液主要差异成分为柚皮苷和新橙皮苷,其中甜橙枳实水煎液中几乎不含柚皮苷和新橙皮苷,而酸橙枳实水煎液中这两种成分含量较高;抗氧化活性结果表明酸橙枳实水煎液抗氧化活性显著优于甜橙枳实水煎液(P <0.05);灰色关联度分析和双变量相关性分析显示,酸橙枳实水煎液中抗氧化作用的核心药效组分是橙皮苷、新橙皮苷、川陈皮素;甜橙枳实水煎液抗氧化作用的核心药效组分是芸香柚皮苷、橙皮素、川陈皮素。结论 本研究建立了HPLC法同时测定枳实水煎液中8种黄酮类成分的含量;不同基原枳实水煎液黄酮类成分含量与抗氧化能力均存在差异。
Objective To compare the differences in flavonoid components and antioxidant activity of water decoctions by Aurantii Fructus Immaturus of different origins,and to provide reference for the quality evaluation and clinical use of Aurantii Fructus Immaturus.Methods HPLC method was used to simultaneously determine 8 flavonoid components(narirutin,naringin,hesperidin,neohesperidin,naringenin,hesperetin,nobiletin and tangeretin)in the water decoction of Aurantii Fructus Immaturus.The differential chemical compositions were screened by multivariate statistics.The antioxidant activities of water decoction from different origins of Aurantii Fructus Immaturus were determined by 1,1-diphenyl-2-picrylhydrazyl(DPPH)method and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)method.The correlation between the content of 8 components and antioxidant activity in the water decoction of Aurantii Fructus Immaturus was evaluated by grey correlation and bivariate correlation analysis.Results The main differential components in the water decoction of different origins of Aurantii Fructus Immaturus were naringin and neohesperidin.There was almost no naringin and neohesperidin in the water decoction of Citrus sinensis,while these two components were higher in the water decoction of Citrus aurantium.The antioxidant activity of the water decoction of Citrus aurantium was significantly better than that of Citrus sinensis(P<0.05).Grey correlation and bivariate correlation analysis showed that the core pharmacodynamic components for the antioxidant effect in the water decoction of Citrus aurantium included hesperidin,neohesperidin and nobiletin;while the core pharmacodynamic components for antioxidant effect in the water decoction of Citrus sinensis were narirutin,hesperetin and nobiletin.Conclusion The HPLC method is established for the determination of 8 flavonoids in the water decoction of Aurantii Fructus Immaturus,with differences in the content of flavonoids and antioxidant capacity in water decoctions of different origins of Aurantii Fructus Immaturus.
作者
赵香玉
熊英
刘永富
卢轩
李宗年
邓可众
ZHAO Xiang-yu;XIONG Ying;LIU Yong-fu;LU Xuan;LI Zong-nian;DENG Ke-zhong(College of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004)
出处
《中南药学》
CAS
2024年第4期967-973,共7页
Central South Pharmacy
基金
国家自然科学基金资助项目(No.81960709)
江西省大学生创新创业计划(No.202210412320)。
关键词
枳实
水煎液
含量测定
抗氧化
多元统计分析
相关性
Aurantii Fructus Immaturus
water decoction
content determination
antioxidant
multivariate statistical analysis
correlation
