摘要
目的:分析化学发光酶免疫分析法(CLIA)诊断乙型肝炎病毒(HBV)感染的价值。方法:选取2022年10月—2023年8月金沙县中医医院收治的疑似乙型肝炎患者60例作为研究对象,均接受酶联免疫吸附法(ELISA)、CLIA及综合检查,以综合检查诊断结果为“金标准”,比较ELISA、CLIA诊断HBV感染的效能。结果:综合诊断结果显示,HBV感染阳性50例,阴性10例。CLIA检测HBV核心抗体、HBV表面抗体、HBV e抗原、HBV e抗体、HBV表面抗原的阳性符合率高于ELISA,差异有统计学意义(P<0.05);CLIA的灵敏度、准确度、阴性预测值高于ELISA,差异有统计学意义(P<0.05);两种检测方法的特异度、阳性预测值比较,差异无统计学意义(P>0.05)。结论:CLIA应用于HBV感染的诊断价值较高,能够稳定检出血清标志物,可为HBV感染的诊断及后续临床治疗工作提供重要依据,值得临床应用并予以推广。
Objective:To analyze the value of chemiluminescence enzyme immunoassay(CLIA)in diagnosing hepatitis B virus(HBV)infection.Methods:A total of 60 suspected hepatitis B patients admitted to Jinsha County Chinese Medicine Hospital from October 2022 to August 2023 were selected as the study subjects.All patients received enzyme-linked immunosorbent assay(ELISA),CLIA and comprehensive examination.The results of comprehensive examination were used as the"gold standard"to compare the efficacy of ELISA and CLIA in diagnosing HBV infection.Results:The comprehensive diagnosis results showed that 50 cases were positive for HBV infection and 10 cases were negative for HBV infection.The positive coincidence rate of HBV core antibody,HBV surface antibody,HBV e antigen,HBV e antibody,HBV surface antigen detected by CLIA was higher than that by ELISA,and the difference was statistically significant(P<0.05).The sensitivity,accuracy and negative predictive value of CLIA were higher than those of ELISA,and the difference was statistically significant(P<0.05).There was no significant difference in the specificity and positive predictive value between the two methods(P>0.05).Conclusion:The application of CLIA in the diagnosis of HBV infection has a high diagnostic value,and can stably detect serum markers,which provides an important basis for the diagnosis and subsequent clinical treatment of HBV infection,and is worthy of clinical application and promotion.
作者
田甜
Tian Tian(Jinsha County Chinese Medicine Hospital,Bijie 551800,Guizhou Province,China)
出处
《中国社区医师》
2024年第9期131-133,共3页
Chinese Community Doctors
关键词
化学发光酶免疫分析法
酶联免疫吸附法
乙型肝炎病毒
Chemiluminescence enzyme immunoassay
Enzyme-linked immunosorbent assay
Hepatitis B virus
