摘要
目的探究针灸百会、足三里穴对慢性温和刺激(chronic unpredictable mild stress,CUMS)诱发抑郁样行为小鼠的抗抑郁效应及其与肠道菌群变化的关联。方法60只小鼠随机分为:对照组、对照+针灸组、CUMS组和CUMS+针灸组。CUMS和CUMS+针灸组进行为期6周的CUMS处理,CUMS处理的后3周对对照+针灸组和CUMS+针灸组小鼠每天进行20 min的百会和足三里穴针灸;使用糖水偏爱和悬尾实验评估小鼠的抑郁样行为;行为结束后取小鼠粪便使用16S rRNA基因测序检测肠道菌群组成。结果单纯针灸对小鼠糖水偏爱和悬尾实验不动时间无影响(P>0.05);CUMS导致小鼠出现抑郁样行为,糖水偏爱率降低(P<0.001)和悬尾不动时间增长(P=0.003);针灸百会和足三里穴使得CUMS+针灸组小鼠相比于CUMS组小鼠糖水偏爱率升高(P=0.035)和悬尾不动时间减少(P=0.045);针灸和CUMS并未导致小鼠肠道菌群丰富度发生变化(P>0.05),但导致肠道菌群组成发生显著变化(P=0.002);CUMS导致肠道Patulibacter等6个菌属丰度升高,Faecalibacterium等26个菌属丰度降低(P<0.05);CUMS+针灸组相比于CUMS组Faecalibacterium等10个菌属丰度升高,Patulibacter等4个菌属丰度降低(P<0.05);四组小鼠有其各自的特征性肠道菌群;CUMS组相比于对照组视黄醇代谢、泛素系统、N-聚糖生物合成和类固醇荷尔蒙生物合成、细胞凋亡和产热作用等基因功能显著富集(P<0.05),而针灸导致CUMS+针灸组小鼠以上肠道菌群基因功能富集程度降低(P<0.05)。结论针灸百会和足三里穴具有抗抑郁效应,且可使抑郁导致的肠道菌群紊乱部分恢复;针灸百会和足三里穴可能通过影响肠道菌群组成及视黄醇、泛素、N-聚糖和类固醇等代谢功能从而发挥抗抑郁效应。
Objective To explore the antidepressant effects of acupuncture at Baihui and Zusanli acupoints on mice with chronic unpredictable mild stress(CUMS)-induced depressive-like behavior and its association with changes in gut microbiota.Methods Sixty mice were randomly divided into four groups:control group(Ctrl),control+acupuncture group(Ctrl+AP),CUMS group,and CUMS+acupuncture group(CUMS+AP).The CUMS and CUMS+AP groups underwent 6-week CUMS treatment.During the last three weeks of CUMS,mice in the Ctrl+AP and CUMS+AP groups received 20 minutes of acupuncture at Baihui and Zusanli acupoints daily.Sucrose preference test and tail suspension test were used to assess the depressive-like behavior in mice.After the behavioral tests,fecal samples were collected from the mice,and the gut microbiota composition was analyzed using 16S rRNA gene sequencing.Results Acupuncture alone had no effect on sucrose preference or tail suspension test results in mice(P>0.05).CUMS induced depressive-like behavior in mice,as evidenced by decreased sucrose preference(P<0.001)and increased immobility time in the tail suspension test(P=0.003).The CUMS+AP group showed increased sucrose preference(P=0.035)and decreased immobility time in the tail suspension test(P=0.045)compared to the CUMS group.Acupuncture and CUMS did not alter the richness of gut microbiota in mice(P>0.05),but they caused significant changes in gut microbiota composition(P=0.002).CUMS increased the abundance of six bacterial genera,including Patulibacter,and decreased the abundance of 26 bacterial genera,including Faecalibacterium(P<0.05).The CUMS+AP group showed increased abundance of 10 bacterial genera,including Faecalibacterium,and decreased abundance of four bacterial genera,including Patulibacter,compared to the CUMS group(P<0.05).Each group of mice had specific gut microbiota.The CUMS group showed significant enrichment of gene functions related to retinol metabolism,ubiquitin system,N-glycan biosynthesis,steroid hormone biosynthesis,apoptosis,and thermogenesis compared to the control group(P<0.05),while acupuncture reduced the enrichment of these gene functions in the CUMS+AP mice(P<0.05).Conclusion Acupuncture at Baihui and Zusanli acupoints has antidepressant effects and can partially restore the depression-induced dysbiosis of gut microbiota.Acupuncture at Baihui and Zusanli acupoints may exert antidepressant effects by influencing gut microbiota composition and metabolism functions of retinol,ubiquitin,N-glycan,and steroids.
作者
田佳荣
孙敦保
孙敦花
朱峰
TIAN Jiarong;SUN Dunbao;SUN Dunhua;ZHU Feng(Department of Psychiatry and Psychology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi’an 710061;Department of Psychiatry,Ankang Central Hospital,Ankang 725000;Department of Psychiatry,Xunyang City Caring Federation Boai Hospital,Ankang 725700,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2024年第3期484-490,共7页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省重点研发计划项目(No.2021GXLH-Z-083)。
关键词
抑郁症
针灸
肠道菌群
百会穴
足三里穴
depression
acupuncture
gut microbiota
Baihui acupoint
Zusanli acupoint
