摘要
青稞是我国青藏高原地区特有的粮食作物,具有良好的改善血糖代谢功效,但其具体降糖活性成分以及它们之间的配伍关系尚未明确。以青稞全粉为研究对象,提取青稞全粉中的多酚、多糖、多肽三种关键活性成分,探究青稞全粉提取物对α-葡萄糖苷酶的抑制能力及其相互作用。通过单因素实验以及Box-Benhnken响应面优化实验,确定了利用微孔板法测定α-葡萄糖苷酶抑制活性的最佳反应体系,即酶浓度0.5 U/mL、缓冲液浓度80 mmol/L、底物浓度5 mmol/L。三种青稞全粉提取物均具有一定的α-葡萄糖苷酶抑制活性,其中青稞多酚的抑制活性最强,α-葡萄糖苷酶活性抑制率达到83.63%±2.92%,对应的IC_(50)值为0.18 mg/mL。三种青稞全粉提取物两两复配均具有一定的协同增效作用。结果可为血糖控制人群的膳食选择和功能性食品的复配开发提供理论指导。
Highland barley is a unique cereal in the Qinghai-Tibet Plateau of China,and has good effects on improving blood glucose metabolism.However,its specific hypoglycemic ingredients and compatibility are not yet clear.In this manuscript,the polyphenols,polysaccharides,and peptides in whole grain highland barley(WHB)were extracted.And theα-glucosidase inhibitory activity of 3 key active ingredients components and their interaction were investigated.The optimal reaction system for measuringα-glucosidase inhibitory activity using the microplate method was first determined through single factor experiments and Box-Benhnken response surface optimization experiments,i.e.,0.5 U/mL of enzyme concentration,80 mmol/L of buffer concentration,and 5 mmol/L of substrate concentration.The results showed that all 3 WHB extracts exhibited goodα-glucosidase inhibitory activity,with WHB polyphenols having the strongest inhibitory activity.Its inhibition rate reached 83.63%±2.92%,corresponding to an IC_(50) of 0.18 mg/mL.In addition,any 2 hypoglycemic ingredients combinations of the 3 WHB extracts had a certain synergistic effect.Our results will provide guidance for dietary choices in blood glucose control populations and new evidence for the development of functional foods.
作者
任欣
刘璇
王补珍
张敏
王林萱
REN Xin;LIU Xuan;WANG Bu-zhen;ZHANG Min;WANG Lin-xuan(National Innovation Center of Grain Industry(Highland Barley Deep Processing),Beijing Engineering and Technology Research Center of Food Additives,College of Food and Health,Beijing Technology and Business University,Beijing 100048,China)
出处
《粮油食品科技》
CAS
2024年第3期132-139,共8页
Science and Technology of Cereals,Oils and Foods
基金
国家自然科学基金项目(32101876)。
关键词
青稞全粉
活性组分
α-葡萄糖苷酶
抑制活性
协同配伍
whole grain highland barley
hypoglycemic active ingredients
α-glucosidase
inhibitory activity
synergistic compatibility
