摘要
目的 探讨福州地区汉族人群线粒体融合蛋白2(Mfn2)基因多态性与超重/肥胖易感性的关系。方法 选择福州地区汉族健康体检者198例,其中体质量正常104例,超重/肥胖94例。采集受试者空腹静脉血,Sanger测序法检测Mfn2基因rs2295281位点的基因型。统计基因位点和基因型频率,采用非条件Logistic回归模型校正混杂因素后,分析不同基因型与超重/肥胖易感性的关系;进一步根据性别进行分层,分析男性、女性人群中不同基因型与超重/肥胖易感性的关系。结果 Mfn2基因rs2295281位点存在CT、TT、CC三种基因型,其中CC基因型携带者78例,CT基因型携带者88例,TT基因型携带者32例;C等位基因频率为61.62%,T等位基因频率为38.38%。Logistic回归分析结果显示,Mfn2基因rs2295281位点的T等位基因较C等位基因超重/肥胖的发病风险增加(P=0.019,OR=1.688,95%CI为1.088~2.618),TT基因型较CC基因型发病风险增加(P=0.018,OR=3.099,95%CI为1.214~7.909),TT基因型较CC+CT发病风险增加(P=0.032,OR=2.572,95%CI为1.086~6.089)。根据性别进行分层统计,男性人群中,T等位基因较C等位基因超重/肥胖的发病风险增加(P=0.043,OR=1.900,95%CI为1.022~3.533),CT基因型较CC基因型发病风险增加(P=0.027,OR=2.803,95%CI为1.123~6.993),CT+TT基因型较CC基因型发病风险增加(P=0.02,OR=2.784,95%CI为1.178~6.584);女性人群中,TT基因型较CC+CT基因型超重/肥胖的发病风险增加(P=0.014,OR=4.683,95%CI为1.366~16.047)。结论 Mfn2基因rs2295281位点的基因分布频率与超重/肥胖易感性存在明显相关性,T等位基因可能增加发病风险,含CT基因的男性人群及含TT基因的女性人群可尽早进行饮食结构调整、运动干预。
Objective To investigate the relationships between mitofusin-2(Mfn 2)gene polymorphism and suscep⁃tibility to overweight/obesity in Han population of Fuzhou.Methods A total of 198 healthy subjects of Han nationality in Fuzhou were selected,of whom 104 were of normal body weight and 94 were overweight/obese.Fasting venous blood was collected and the genotypes of rs2295281 locus of Mfn2 gene were detected by Sanger sequencing.The frequency of gene loci and genotype were counted.After adjusting the confounding factors,the relationships between different geno⁃types and susceptibility to overweight/obesity were analyzed by unconditioned Logistic regression model.Further stratifica⁃tion by sex was conducted to analyze the relationships between different genotypes and overweight/obesity susceptibility in male and female populations.Results There were three genotypes(namely CT,TT,and CC)in the rs2295281 site of Mfn2 gene,including 78 cases of CC genotype,88 cases of CT genotype and 32 cases of TT genotype.The frequency of C allele was 61.62%and the frequency of the T allele was 38.38%.The results of the Logistic-regression analysis showed that, the T allele at the rs2295281 locus of the Mfn 2 gene had an increased risk of developing overweight/obesity in com⁃ parison with the C allele (P=0. 019, OR=1. 688, 95% CI 1. 088-2. 618), the TT genotype had an increased risk in com⁃ parison with the CC genotype (P=0. 018, OR=3. 099, 95% CI 1. 214-7. 909), the TT genotype also had an increased risk in comparison with CC + CT genotype (P=0. 032, OR=2. 572, 95% CI 1. 086-6. 089). Stratified statistics based on gen⁃ der found that, in males, T allele was associated with an increased risk of overweight/obesity in comparison with C allele (P=0. 043, OR=1. 900, 95%CI 1. 022-3. 533), CT genotype was associated with an increased risk in comparison with CC genotype (P=0. 027, OR=2. 803, OR=2. 803), and CT + TT genotype had a higher risk than CC genotype (P=0. 02, OR= 2. 784, 95%CI 1. 178-6. 584);in females, TT genotype was associated with an increased risk of overweight/obesity in comparison with CC + CT genotype (P=0. 014, OR=4. 683, 95% CI 1. 366 to 16. 047). Conclusions There is an obvi⁃ ous correlation between the gene distribution frequency of the rs2295281 locus of Mfn 2 gene and the susceptibility to over⁃ weight/obesity. The T allele may increase the risk, and men with CT gene and women with TT gene can have diet and exer⁃ cise intervention as early as possible.
作者
黄惠娟
俞烜华
张富
梁玲
李淼鋆
陈孔敏
黄文金
HUANG Huijuan;YU Xuanhua;ZHANG Fu;LIANG Ling;LI Miaoyun;CHEN Kongmin;HUANG Wenjin(Department of Preventive Treatment of Disease,The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350004,China;不详)
出处
《山东医药》
CAS
2024年第14期22-26,共5页
Shandong Medical Journal
基金
福建省中青年教师教育科研项目(科技类)立项课题(JAT200188)。
