摘要
为提示广西地区番鸭源鹅细小病毒(Muscovy duck-origin goose parvovirus,MDGPV)的分子流行病学特征及其遗传变异机理,本研究采用PCR方法对某番鸭场送检的病料进行病原鉴定,将PCR阳性样品接种于12~13日龄番鸭胚尿囊腔进行病毒的分离,对分离到的毒株进行基因扩增、克隆和测序,并与参考株序列进行比较分析,绘制系统遗传进化树。结果成功分离到一株MDGPV毒株,命名为GXBH株。该分离株与GenBank中17株水禽细小病毒的同源性为79.4%~98.7%,其中与鹅细小病毒Goose parvovirus(GPV)参考毒株的核苷酸同源性较高,在93.3%~98.7%之间;与番鸭细小病毒(MDPV)参考毒株的同源性较低,在79.4%~79.8%之间;与MDPV参考毒株处于进化树的不同分支,而与GPV参考毒株处于同一进化分支。本研究分离毒株为番鸭源鹅细小病毒,与GPV代表毒株具有相近的遗传演化关系。
In order to reveal the molecular epidemiological characteristics of a goose parvovivus from muscovy in Guangxi region and its genetic variation mechanism.In this study,PCR was used to identify the pathogen of the disease materials sent for inspection by a muscovy duck farm;the identified positive samples were inoculated into allantoic cavity of 12~13 day-old muscovy duck embryos to isolate the virus.The gene was amplified,cloned and sequenced;and the gene sequences of reference strains were compared and analyzed to construct a phy-logenetic tree.The results showed that Muscovy duck-origin goose parvovirus(MDGPV)was isolated successfully and named as GXBH strain;the homology between this isolation and 17 isolates of waterfowl parvovirus in GenBank was 79.4%~98.7%;The isolated strain had higher homology with the representative strains of GPV,while it had lower homology with the representative strains of MDPV.The gene shared 93.3%~98.7%nucleotide homology to Goose parvovirus(GPV)strains and 79.4%~79.8%nucleotide homology to Muscovy duck parvorirus(MDPV).It was in a different branch of the evolutionary tree with the MDPV strains,and was in the same branch with the GPV strains.The results suggested that the isolated strain was MDGPV,which had similar genetic evolutionary relationships with the represen-tative strains of GPV.
作者
何奇松
马琳
马军
严悌昆
胡丽萍
黄胜斌
何丹
蓝惠华
韩银华
周庆安
黄张玲
熊毅
HE Qisong;MA Lin;MA Jun;YAN Tikun;HU Liping;HUANG Shengbin;HE Dan;LAN Huihua;HAN Yinhua;ZHOU Qingan;HUANG Zhangling;XIONG Yi(Guangxi Center for Animal Disease Prevention and Control,Nanning 530004,China;Guangdong Yuyue Biotechnology Company,Guangzhou 510535,China;Nanning Center for Animal Disease Prevention and Control,Nanning 530001,China)
出处
《特产研究》
2024年第3期42-46,共5页
Special Wild Economic Animal and Plant Research
基金
广西农业农村厅科技项目(Z2022192、Z202034)。
关键词
番鸭
鹅细小病毒
分离
鉴定
muscovy duck
goose parvovirus
isolation
identification
