摘要
为了快速检测犬类动物呼吸道和消化道细菌的分布状况并研究其特性,选取南京警犬研究所部分患病警犬为试验对象,收集呼吸道、肠道分泌液,使用TaqMan探针-荧光定量PCR技术进行扩增鉴定,分离细菌后进行药敏试验、细菌侵染细胞试验并检测凋亡相关斑点样蛋白(ASC)的变化。结果表明:试验犬呼吸道内以β-内酰胺酶抗性基因菌、金黄色葡萄球菌、无乳链球菌为主要细菌,消化道内以大肠杆菌、β-内酰胺酶抗性基因菌、支原体为主流细菌;药敏试验表明肺炎克雷伯氏菌对头孢类抗生素敏感,无乳链球菌对多种抗生素敏感,经过无乳链球菌的侵染后的细胞凋亡率为4.08%,而经过肺炎克雷伯氏菌侵染后的细胞凋亡率为13.47%,侵染细胞后0~4 h炎症因子ASC显著上升。综上,TaqMan探针-荧光定量PCR技术可以快速检测出警犬呼吸道和肠道细菌,筛选出患犬呼吸道和肠道的主流菌,这些细菌对头孢类等抗生素敏感,可为兽医针对性地用药提供参考。
To rapidly detect the distribution of bacteria in the respiratory tract and digestive tract of dogs and to study their infection mecha⁃nisms,we selected some sick police dogs in Nanjing Police Dog Institute,collected they respiratory secretions and intestinal secretions,and used the TaqMan probe-fluorescence quantitative PCR technology to amplify and identify the types of pathogenic bacteria in the secretions.Then,the identified pathogens were isolated to test their drug sensitivity.Bacteria infection experiments were carried out on cells,and the changes in apoptosis-associated speck-like proteins containing a CARD(ASC)in cells were detected.The PCR results showed thatβ-lacta⁃mase resistance bacteria,Staphylococcus aureus and Streptococcus agalactis were the main pathogens in the respiratory tract of the police dogs.Escherichia coli,β-lactamase resistance bacteria and mycoplasma were the main stream bacteria in the digestive tract.The results of the drug sensitivity test showed that Klebsiella pneumoniae was sensitive to cephalosporin antibiotics,and Streptococcus agalactia was sensitive to many antibiotics.After the infection of Streptococcus agalactis,the pyrogenic cells accounted for 4.08%of the total cells.The pyrogenic cells infec⁃ted by Klebsiella pneumoniae accounted for 13.47%of the total cells.The inflammatory factor ASC increased within 0-4 h after infection.This experiment indicated that the TaqMan probe-fluorescent quantitative PCR technique was able to quickly detect the bacteria in the respir⁃atory tract and intestinal tract of police dogs,the main bacteria in the respiratory truct and intestinal tract of sick polics dogs were screened out,those bacteria were sensitive to cephalosprin etc.Theresults helped to understand the infection mechanism,and provided targeted medi⁃cation advice for veterinarians.
作者
蔡靖
王苏苏
李涛
丁宁
王莹
王志鹏
王子渲
包喜军
朱程程
蔡亚非
CAI Jing;WANG Susu;LI Tao;DING Ning;WANG Ying;WANG Zhipeng;WANG Zixuan;BAO Xijun;ZHU Chengcheng;CAI Yafei(Police Dog Technology Institute,Nanjing Police University,Nanjing 210012,China;College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Dairy Herd Improvement Center,Nanjing 210095,China;Guangzhou Customs Technical Center,Guangzhou 510623,China)
出处
《畜牧与兽医》
CAS
北大核心
2024年第6期110-117,共8页
Animal Husbandry & Veterinary Medicine
基金
公安部科技强警基础工作专项(2021JC26)。
关键词
病警犬细菌
PCR快速检测
药物敏感性
炎症因子ASC
bacteria in sick police dogs
rapid detection by PCR
susceptibility to drugs
inflammatory factor ASC
