摘要
目的研究Diaphanous相关成蛋白3(DIAPH3)对细胞毒素相关蛋白A(cagA^(+))幽门螺杆菌(Hp)诱导的胃黏膜上皮GES-1细胞上皮间质转化(EMT)及紧密连接蛋白表达的影响。方法H.pylori J99毒株(cagA^(+)Hp)与GES-1细胞株共培养,使用DIAPH3干扰片段对细胞进行干预。观察细胞增殖、侵袭、凋亡情况,并检测EMT相关蛋白和紧密连接蛋白变化。结果显微镜下显示cagA^(+)Hp共培养的GES-1细胞大小不一,形态发生异变。与对照组比较,模型组、DIAPH3-NC组和DIAPH3组干预后不同时间的OD_(570)值、侵袭细胞数量均增加,凋亡率降低,N钙黏附蛋白(N-cadherin)、波形蛋白(Vimentin)相对表达量升高,E-钙黏附蛋白(E-cadherin)和紧密连接蛋白(occludin、claudin-1、ZO-1)相对表达量降低(P<0.05);与模型组、DIAPH3-NC组比较,DIAPH3组的OD_(570)值、侵袭细胞数量均降低,凋亡率增加,N-cadherin、Vimentin相对表达量降低,E-cadherin、occludin、claudin-1、ZO-1相对表达量升高(P<0.05)。结论抑制NIAPH3表达能抑制胃黏膜上皮GES-1细胞的EMT,阻止细胞的侵袭和转移,提高凋亡率,上调紧密连接蛋白表达。
Aim To investigate the effect of diaphanous-related formin 3(DIAPH3)on epithelial to mesenchymal transformation(EMT)and tight junction protein expression in gastric epithelial GES-1 cells induced by cytotoxin-related protein(cagA^(+))and Helicobacter pylori(Hp).Methods H.pylori J99 strain(cagA^(+)Hp)was co-cultured with GES-1 cell line.The cell proliferation,invasion and apoptosis were observed,and the changes of interstitial transition(EMT)related proteins and tight junction proteins were detected.Results Compared with the control group,the OD_(570)value and the number of invasive cells in the model,DIAPH3-NC,and DIAPH3 groups increased at different times after intervention,and apoptosis rate decreased.The relative expressions of N-cadherin and Vimentin increased.The relative expression of E-cadherin and tight junction proteins(E-Cadherin,claudin-1,ZO-1)decreased(P<0.05);Compared with the model group and the DIAPH3-NC group,the OD_(570)value,the number of invasive cells,apoptosis rate,and the relative expression levels of N-cadherin and Vimentin decreased in the DIAPH3 group.The relative expression of E-cadherin,occludin,claudin-1 and ZO-1 increased(P<0.05).Conclusion Inhibition of NIAPH3 expression can inhibit EMT in gastric epithelium GES-1 cells,prevent cell invasion and metastasis,increase apoptosis rate,and up-regulate the expression of tight junction protein.
作者
黎振东
毛苇
LI Zhendong;MAO Wei(Department of Gastroenterology,Hainan Hospital Affiliated to Hainan Medical College,Haikuo 570000,Hainan,China)
出处
《中南医学科学杂志》
CAS
2024年第3期364-367,371,共5页
Medical Science Journal of Central South China
