摘要
银缕梅是我国特有的珍稀濒危植物,据连续多年的调查,该物种受炭疽病的危害较为严重。本文在样品采集、病原菌分离和致病性鉴定的基础上,利用形态学和分子生物学手段,明确了银缕梅炭疽病病原菌的种类。结果表明:从病叶中分离到1株病原真菌,命名为YLMJ,该真菌可侵染银缕梅叶片,发病症状与野外发病时一致。形态学鉴定结果显示:病原菌在培养基上呈放射状生长,初期菌落为白色,后变为深褐色,菌丝细长、绒毛状、多锐角分支,形态与尖孢炭疽菌相似。序列比对结果表明:YLMJ的内转录间隔区、肌动蛋白基因、β-微管蛋白基因序列与尖孢炭疽菌的同源性最高,在系统发育树上均聚于同一分支。结合形态学与多基因联合鉴定结果,明确银缕梅炭疽病的病原菌为尖孢炭疽菌。银缕梅炭疽病病原菌的分离和鉴定,为后续开展病害的科学防治与致病机理研究奠定了基础。
Parrotia subaequalis is a rare and endangered plant species endemic to China.Years of investigation have shown that this species is affected by anthracnose disease.Based on sample collection,pathogenic fungus isolation,and pathogenicity identification,this study employed morphological and molecular biological methods to determine the type of pathogenic fungus causing P.subaequalis anthracnose disease.The results showed that one strain of pathogenic fungus was isolated from diseased leaves and named YLMJ.This fungus can invade Parrotia subaequalis leaves,causing symptoms consistent with those observed in the wild.Morphological identification revealed that the pathogenic fungus grew radially on culture media,with initial white colonies turning into dark brown later.The hyphae were thin and filamentous with multiple sharp-angled branches,which was similar in appearance to Colletorichum acutatum.Sequence alignment results indicated that YLMJ had the highest homology with C.acutatum in internal transcribed spacer,actin gene and beta-tubu-lin gene sequences,and they clustered in the same clade on phylogenetic trees.Combining morphological and multi-gene identification results confirmed that the pathogenic fungus causing P.subaequalis anthracnose disease was C.acutatum.Isolation and identification of the pathogenic fungus causing P.subaequalis anthracnose disease laid the foundation for subsequent scientific prevention of the disease and research on its pathogenic mechanism.
作者
田盛野
杨乐乐
潘伟伟
张焱琴
顾航
张慧娟
蒋明
Tian Shengye;Yang Lee;Pan Weiwei;Zhang Yanqin;Gu Hang;Zhang Huijuan;Jiang Ming(School of Life Sciences,Taizhou University,Taizhou 318000,China;College of Life Sciences,Hunan Normal University,Changsha 410000,China)
出处
《台州学院学报》
2024年第3期23-28,共6页
Journal of Taizhou University
基金
浙江省大学生科技创新活动计划暨新苗人才计划项目(2022R436A006)。
关键词
银缕梅
炭疽病
鉴定
尖孢炭疽菌
Parrotia subaequalis
anthracnose disease
identification
Colletorichum acutatum
