摘要
为了提高忍冬桑黄总黄酮的提取率,以忍冬桑黄(Sanghuangporus lonicericola)子实体为原料,采用超声波辅助乙醇提取其总黄酮,以料液比、乙醇体积分数、超声时间和超声温度进行单因素试验,通过响应面试验对忍冬桑黄总黄酮提取工艺进行优化。结果表明,忍冬桑黄子实体总黄酮最优提取工艺:料液比1∶60、乙醇体积分数60%、超声时间60 min、超声温度76℃。在最佳工艺条件下,忍冬桑黄子实体总黄酮提取率为0.80%,与模型预测值0.83%接近,表明该提取条件合理可行,可以提高忍冬桑黄总黄酮提取率,利于忍冬桑黄产品精深加工及桑黄资源的综合利用。
In order to improve the extraction rate of total flavonoids from Sanghuangporus lonicericola,this study used the fruiting body of S.lonicericola as the raw material,and used ultrasound assisted ethanol extraction to extract its total flavonoids.The extraction process was optimized by response surface testing,which was conducted based on single factor tests of liquid⁃solid ratio,ethanol concentration,ultrasonic time,and ultrasonic temperature.The optimal extraction process of total flavonoids from the fruit bodies of S.lonicericola was as follows:solid⁃liquid ratio of 1∶60,ethanol concentration of 60%,ultrasonic time of 60 min and ultrasonic temperature of 76℃.In the optimal conditions,the extraction rate of total flavonoids from S.lonicericola was 0.80%,which was close to the predicted value of 0.83%of the model,indicating that the optimized process is reasonable and feasible,can improve the extraction rate of total flavonoids from S.lonicericola,and facilitate the deep processing of S.lonicericola products and the comprehensive utilization of Sanghuang resources.
作者
杨娟娟
孙波
王卓仁
刘启燕
赵会长
周洪英
常菊花
YANG Juanjuan;SUN Bo;WANG Zhuoren;LIU Qiyan;ZHAO Huizhang;ZHOU Hongying;CHANG Juhua(School of Life Sciences,Changjiang University,Jingzhou 434025,China;Economic Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)
出处
《河南农业科学》
北大核心
2024年第5期172-180,共9页
Journal of Henan Agricultural Sciences
基金
国家自然科学青年基金项目(31600010)
湖北省重点研发计划项目(2021BBA245)
国家蚕桑产业技术体系武汉试验站项目(CARS-18-SYZ11)
湖北省食用菌产业技术体系项目(HBHZDZB-2021-023)。
关键词
忍冬桑黄
总黄酮
超声波辅助乙醇提取
响应面试验
提取工艺
Sanghuangporus lonicericola
Total flavonoids
Ultrasonic assisted ethanol extraction
Response surface test
Ectraction process
