摘要
目的:探讨抗原修复时间对不同克隆号的P40免疫组化染色结果的影响。方法:选择本科室P40免疫组化染色异常的胸水细胞沉渣包埋蜡块,3μm连续切片4张,按抗原修复时间不同分为两组(A组抗原修复40 min,B组抗原修复80 min),每组两张切片分别滴加C3B4和MXR010两种不同克隆号的P40抗体,进行免疫组化染色对比实验。结果:A组的C3B4出现胞浆胞膜中度非特异染色,而MXR010没有出现非特异染色。B组的C3B4出现胞浆胞膜高度非特异染色,而MXR010出现胞浆胞膜轻度非特异染色。同一克隆号,A组和B组相比,随着抗原修复时间的延长,MXR010从无非特异染色变为轻度非特异染色,而C3B4从中度非特异染色加重为高度非特异染色。结论:抗原修复时间对不同克隆号的P40免疫组化染色结果会产生影响,随着抗原修复时间的延长,越容易出现非特异染色。选择适当的抗原修复时间和特异性好的抗体,可以减少免疫组化的非特异染色。
Objective:To explore the effect of antigen retrieval time on the immunohistochemical staining results of P40 with different Clones.Methods:The pleural effusion cell sedimen with abnormal immunohistochemical staining of P40 was collected.Four sections of cell sediment with a thickness of 3 microns were divided into two groups according to antigen retrieval time.group A had a duration of 40 minutes and group B had a duration of 80 minutes.Performed immunohistochemical staining and added P40 antibodies of Clone C3B4 and MXR010 to two slices separately in each group.Results:In group A,C3B4 showed moderatenonspecific staining of cytoplasm and membrane,while MXR010 did not show nonspecific staining.In group B,C3B4 showed severe nonspecific staining of cytoplasm and membrane,while MXR010 showed mildnonspecific staining of cytoplasm and membrane.Compared the same clone between group A and group B,with the prolongation of antigen retrieval time,MXR010 changed from no nonspecific staining to mild nonspecific staining,while C3B4 changed from moderate nonspecific staining to severe nonspecific staining.Conclusion:The antigen retrieval time will have an effect on the immunohistochemical staining results of P40 with different clones.As the antigen retrieval time prolongs,nonspecific staining becomes more likely to occur.Choosing appropriate antigen retrieval time and antibodies with good specificity can reduce nonspecific staining.
作者
陈贵东
杨通
沈艳
古婉仪
CHEN Guidong;YANG Tong;SHEN Yan;GU Wanyi(Department of Pathology,Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 511447,Guangdong,China)
出处
《广州医科大学学报》
2024年第2期51-55,共5页
Academic Journal of Guangzhou Medical University
关键词
免疫组化
抗原修复时间
P40
immunohistochemistry
antigen retrieval time
P40
