摘要
为了解植物油脂肪酸积累通路中关键基因GPAT在大豆脂质合成中的作用,文章通过生物信息学方法对大豆GPAT基因家族进行鉴定和表达分析。结果表明,大豆全基因组中共鉴定获得89个GmGPAT基因,分布在20条染色体上。系统进化分析表明大豆GPAT基因家族的多样性和功能分化与拟南芥和蒺藜苜蓿基因家族演化趋势相似。GmGPAT基因家族结构具有复杂性和多样性,不同基因在调控和功能上存在差异,该基因启动子含有多种逆境胁迫和激素响应调控元件。基因家族共线性分析表明不同植物基因组具有相似性和差异性。GmGPAT表达模式可分为4簇,第I簇中各基因在花芽中表达量相对较高,第Ⅳ簇中各基因在籽粒中表达量相对较高,表明该基因可能与花芽和籽粒的发育调控相关。
In order to understand the role of GPAT,a key gene in fatty acid accumulation pathway in vegetable oils,in soybean lipid synthesis,the soybean GPAT gene family was identified and its expression was analyzed by bioinformatics methods.The results showed that a total of 89 GmGPAT genes were identified in the whole soybean genome,distributed on 20 chromosomes.Phylogenetic analysis showed that the diversity and functional differentiation of soybean GPAT gene family was similar to that of Arabidopsis and Tribulus alfalfa.The GmGPAT gene family structure was complex and diverse,and different genes may have differences in regulation and function.The gene promoter contained various regulatory elements of stress and hormone response.The collinearity analysis of gene families showed that the genomes of different plants had similarities and differences.Gene expression pattern analysis showed that GmGPAT expression pattern was divided into four clusters.Gene expression level of clusterⅠwas higher in flower bud,gene expression level of cluster IV was higher in grain,indicated that this gene might be involved in the formation of flower bud and grain.
作者
吴苏峻
王宇
王艳丽
张锴
乔亚科
WU Sujun;WANG Yu;WANG Yanli;ZHANG Kai;QIAO Yake(College of Agronomy and Biotechnology,Hebei Normal University of Science&Technology/Hebei Key Laboratory of Crop Stress Biology,Qinhuangdao 066000,Hebei,China;College of Horticulture Science and Technology,Hebei Normal University of Science&Technology,Qinhuangdao 066000,Hebei,China)
出处
《大豆科技》
2024年第3期7-15,共9页
Soybean Science & Technology
基金
河北科技师范学院科学研究基金项目(304020125)
河北省科技厅现代种业科技创新专项项目(21326313D)。
