摘要
目的研究缺血性脑卒中患者血清差异基因的筛选及生物信息学。方法以2023年3月-2024年3月在新疆医科大学第二附属医院神经内科确诊的80例缺血性脑卒中患者为病例组,选择同期80例健康体检者为对照组。分别挑选两组各10例受试者的外周血清采用芯片差异性基因鉴定法筛选缺血性脑卒中差异表达的长链非编码RNA(lncRNA),并采用KEGG通路富集和基因本体论(GO)分析鉴定差异表达基因发挥的生物学功能。挑选2个上调和2个下调的lncR-NAs,在两组患者外周血中采用实时荧光定量PCR(qRT-PCR)法检测表达量,采用受试者工作特征曲线(Receiver operating characteristic,ROC)计算差异性表达lncRNAs诊断缺血性脑卒中的曲线下面积(Area under the curve,AUC)。结果共检测到34个高表达和16个低表达的lncR-NAs。KEGG通道分析显示,差异表达的lncRNAs涉及肿瘤坏死因子(TNF)信号通路、类风湿性关节炎、细胞因子与细胞因子受体相互作用,病毒蛋白与细胞因子和细胞因子受体的相互作用、癌症的转录失调、沙门氏菌感染、白细胞介素(IL)-17信号通路、趋化因子信号通路。GO分析显示,差异表达的lncRNAs涉及白细胞黏附调控、细胞黏附调节、白细胞与其他细胞黏附、细胞趋化性、T细胞活化、骨髓细胞分化、止血和凝血。qRT-PCR检测显示,与对照组比较,病例组患者A1BG-AS1和BRWD1-AS2表达量升高,BVES-AS1和C10ORF71-AS1表达量降低,差异有统计学意义(P<0.05)。ROC分析显示,A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1表达量诊断缺血性脑卒中的AUC分别为0.803、0.856、0.897和0.798(P<0.001)。结论缺血性脑卒中患者外周血中A1BG-AS1、BRWD1-AS2、BVES-AS1和C10ORF71-AS1基因差异性表达,可以辅助缺血性脑卒中的疾病诊断。
Objective To analyze screening and bioinformatics of serum differentially expressed genes in pa-tients with ischemic stroke.Methods 80 patients with ischemic stroke diagnosed by the hospital from March 2023 to March 2024 were included as case group,and 80 healthy volunteers were selected as control group during the same period.2 groups of 10 subjects were selected,then peripheral blood microarray chip technology was used to identify significantly differentially expressed long chain non coding RNAs(lncR-NAs)of ischemic stroke.KEGG pathway enrichment and gene ontology(GO)analysis were used to iden-tify biological functions.The research selected 2 upregulated and 2 downregulated lncRNAs,detected their expression levels in the peripheral blood of both groups of the patients using real-time fluorescence quantitative PCR(qRT-PCR).The receiver operating characteristic(ROC)curve was used to calculate the area under the curve(AUC)for diagnosing ischemic stroke using significantly differentially expressed ln-cRNAs.Results A total of 34 significantly high expressed and 16 significantly low expressed lncRNAs.KEGG channel analysis showed that differentially expressed lncRNAs was related to tumor necrosis factor(TNF)signaling pathway,rheumatoid arthritis,cytokine-cytokine receptor interactions,viral protein-cy-tokine receptor interactions,cancer transcriptional dysregulation,salmonella infection,interleukin-17 sig-naling pathway and chemokine signaling pathway.GO analysis showed that differentially expressed lncR-NAs was related to leukocyte adhesion regulation,cell adhesion regulation,leukocyte adhesion to other cells,cell chemotaxis,T cell activation,and significantly downregulated bone marrow cell differentiation,hemostasis and coagulation.qRT-PCR detection showed that compared to control group,the expression levels of A1BG-AS1 and BRWD1-AS2 in the case group were significantly higher,the expression levels of BVES-AS1 and C10ORF71-AS1 were significantly lower(P<0.05).ROC analysis showed that the AUC values for diagnosing ischemic stroke were 0.803,0.856,0.897 and 0.798(P<0.001)by A1BG-AS1,BRWD1-AS2,BVES-AS1 and C10ORF71-AS1 expression levels,respectively.Conclusion The differen-tial expressions of A1BG-AS1,BRWD1-AS2,BVES-AS1 and C10ORF71-AS1 genes in the peripheral blood of the patients with ischemic stroke can assist diagnosis of ischemic stroke.
作者
王洋
汪露
李辉
WANG Yang;WANG Lu;LI Hui(Department of Clinical Laboratory,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830002,China)
出处
《新疆医科大学学报》
CAS
2024年第6期817-823,共7页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区重点实验室基金项目(XJDX1711-2233)。
关键词
缺血性脑卒中
微阵列芯片
高通量测序
长链非编码RNA
ischemic stroke
microarray chip
high throughput sequencing
long chain non coding RNA(lncRNAs)
