摘要
[目的]‘玉露香梨’果实萼片宿存严重降低其商品价值,筛选并鉴定其果实萼片发育关键基因,可为从分子水平培育萼片脱落的梨果实提供参考基因。[方法]以棚架栽培的‘玉露香梨’为试材,结合前期BSA基因定位测序数据,对候选基因表达特征进行验证、克隆并分析2个生长素酰胺合成酶基因GH3家族生物信息特征。[结果]基因PbGH3.3(Pbr007550)和PbGH3.4(Pbr030571)在萼片的表达量排名第二;PbGH3.3和PbGH3.4 CDS长度均为1806 bp,编码601个氨基酸,其中二者存在7个氨基酸序列的差异,编码的蛋白PbGH3.3和PbGH3.4序列与苹果MdGH3.1的同源性最高;属于不稳定性蛋白,无信号肽的存在,定位于细胞质;利用NCBI网站CCD工具分析发现均具有GH3蛋白家族的GH3 superfamliy保守结构域,蛋白质三级建模后发现为GH3.1 AUXIN缀合酶,涉及生长素稳态,属于GH3蛋白家族。STRING互作网络蛋白成员GO和KEGG功能富集分析表明PbGH3.3参与植物激素尤其是生长素信号通路,推测PbGH3.3和PbGH3.4可能在‘玉露香梨’果实萼片脱落中通过IAA信号通路发挥生物学功能。
[Objective]The persistent sepals of‘Yuluxiangli’pear fruits significantly reduces its commercial value.This study aimed to screen and identify key genes involved in sepal development to provide reference genes for molecular breeding of pear fruits with sepal abscission at the molecular level.[Method]‘Yuluxiangli’cultivated in vertical trellis was used as tested mate-rial.Based on previous BSA gene mapping and sequencing data,the expression characteristics of candidate genes were verified,and two auxin-amido synthetase genes,GH3 family,were cloned and analyzed for their bioinformatics characteristics.[Result]The genes PbGH3.3(Pbr007550)and PbGH3.4(Pbr030571)ranked second in expression level in the sepals.The CDS lengths of PbGH3.3 and PbGH3.4 were both 1806 bp,encoding 601 amino acids.There were seven amino acid sequence dif-ferences between them,and the encoded protein PbGH3.3 and PbGH3.4 showed the highest homology with MdGH3.1 in ap-ple.They were unstable proteins without signal peptides and were located in the cytoplasm;Analysis using the CCD tool on the NCBI website revealed that they both possessed the conserved domain GH3 superfamily of GH3 protein family.Protein ter-tiary structure modeling showed that they were GH3.1 Auxin amido synthetase involved in auxin homeostasis,belonging to the GH3 protein family.Functional enrichment analysis of GO and KEGG pathways of STRING protein interaction network mem-bers indicated that PbGH3.3 is involved in the plant hormones,especially auxin signaling pathway.[Conclusion]It was sug-gested that PbGH3.3 and PbGH3.4 might play a biological roles in the regulating of sepal in‘Yuluxiangli’fruits through the IAA signaling pathway.
作者
丁保朋
李小白
林雨静
胡朝慧
杨茹
韦博霖
曹一凡
白钰琳
闫如玉
李六林
Ding Baopeng;Li Xiaobai;Lin Yujing;Hu Chaohui;Yang Ru;Wei Bolin;Cao Yifan;Bai Yulin;Yan Ruyu;Li Liulin(Engineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education and Key Laboratory of National Forest and Grass Administration for the Application of Graphene in Forestry,Shanxi Da-tong University,Datong 037009,China;College of Horticulture,Shanxi Agricultural University,Jinzhong 030801,Chi-na;Datong comprehensive inspection and testing center,Datong 037009,China)
出处
《山西农业大学学报（自然科学版）》
CAS
北大核心
2024年第3期14-23,共10页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
国家自然科学基金青年基金项目(32102364)。
