摘要
探究不同海拔梯度云南松根际土壤及根内细菌种类、群落结构组成及多样性,为研究云南松在不同海拔条件下的适应机制提供一定的微生物学角度的证据。采用高通量测序技术对不同海拔云南松根和根际土壤细菌16S rRNA基因V5~V7区域进行测序,并对其群落结构组成、多样性以及功能进行分析。结果显示:(1)通过高通量测序分析,云南松根内生细菌共鉴定出41门118纲277目465科893属1755种,而根际土壤细菌共鉴定出34门96纲230目395科761属1626种。(2)云南松根际土壤细菌群落香农指数显著高于根内,并且根际土壤中细菌丰度最高的为变形菌门,而根内丰度最高的为放线菌门,根内放线菌门和厚壁菌门相对丰度是根际土壤的2倍,而根际土壤中酸杆菌门相对丰度是根内的2倍,此外根内生细菌群落代谢功能丰度相较于根际土壤细菌较高。(3)云南松根际土壤和根内生细菌群落在不同海拔梯度间的β-多样性存在显著差异(P<0.05),此外不同海拔梯度之间优势物种相对丰度占比存在差异。PICRUSt2功能预测表明,根际土壤细菌基因二级功能表达量随海拔梯度增加而逐渐增加,并且根内生细菌基因功能表达量仅在海拔2900 m处较高。可得结论:不同海拔梯度下云南松根际土壤与根内生细菌多样性、细菌群落的环境适应、功能上均存在差异,这可能与云南松对外界环境的适应有关。本研究为进一步探究云南松的环境适应机制提供了一定的数据支撑。
The species,community structure and diversity of bacteria in rhizosphere soil and roots of Pinus yunnanensis at different altitudes were studied to provide some microbial evidence for the study of the adaptive mechanism of P.yunnanensis at different altitudes.The bacterial 16S rRNA gene v5-v7 region of P.yunnanensis roots and rhizosphere soils at different elevations was sequenced by high-throughput sequencing technology,and the community structure,diversity and function were analyzed.Based on high-throughput sequencing analysis,41 phyla,118 classes,277 orders,465 families,893 genera,1755 species of endophytic bacteria were identified from the roots of P.yunnanensis,and 34 phyla,96 classes,230 orders,395 families,761 genera were identified from the rhizosphere soil,1626 species.The Shannon Index of bacterial community in the rhizosphere soil of P.yunnanensis was significantly higher than that in the root.The most abundant bacteria in the rhizosphere soil were Proteobacteria and the most abundant Actinomycetes in the root,the relative abundance of Actinomycetes and Firmicutes in Roots was twice that in rhizosphere soil,and the relative abundance of Acidobacteria in rhizosphere soil was twice that in roots,in addition,the metabolic functional abundance of endophytic bacteria community in root was higher than that in rhizosphere soil.Theβ-diversity of rhizosphere soil and root endophytic bacterial community of P.yunnanensis was significantly different at different altitudes(P<0.05),and the relative abundance of dominant species was different at different altitudes.The function prediction of PICRUST2 showed that the gene expression level of rhizosphere bacteria increased with the elevation gradient,and the gene expression level of root endophytic bacteria was only high at 2900 m.In conclusion,the diversity of endophytic bacteria in the rhizosphere soil and roots of P.yunnanensis was studied under different altitude gradients,this may be related to the adaptation of P.yunnanensis to the external environment.This study provides some data support for further exploring the environmental adaptation mechanism of P.yunnanensis.
作者
普蕾
周宇杨
和滢埝
母德锦
华国莉
陈林
陈诗
PU Lei;ZHOU Yuyang;HE Yingnian;MU Dejin;HUA Guoli;CHEN Lin;CHEN Shi(Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China,Southwest Forestry University,Kunming Yunnan 650224,P.R.China)
出处
《西部林业科学》
CAS
北大核心
2024年第3期133-143,共11页
Journal of West China Forestry Science
基金
云南省“兴滇英才支持计划”青年人才专项(XDYC-QNRC-2022-0250)
云南省科技厅应用基础研究项目(202201AT070023)
云南省研究生导师团队建设项目(2022-97)
云南省教育厅研究生科研创新项目(2022Y586)。
关键词
云南松
海拔
细菌群落
多样性
高通量测序
Pinus yunnanensis
altidude
bacterial community
diversity
high-throughput sequencing
