摘要
目的对比荧光定量核酸扩增检测技术与环介导等温扩增技术在呼吸道感染病原体检测中的应用价值。方法随机在2020年1月~2023年1月天津市河东区疾病预防控制中心接收的呼吸道感染的患者中选取120例作为研究对象,分别采用荧光定量核酸扩增(PCR)检测技术与环介导等温扩增技术(LAMP)检测呼吸道病原体,并对检测结果进行比较分析。结果120例患者中使用LAMP检出细菌感染85例(阳性率为70.83%)。荧光定量PCR检出细菌感染72例(阳性率60.00%),差异无统计学意义(P>0.05);其中LAMP法对耐甲氧西林葡萄球菌、肺炎链球菌、流感嗜血杆菌的检出率高于荧光定量PCR法,差异有统计学意义(P<0.05);采用荧光定量PCR与LAMP法对呼吸道感染10种病原体进行检测,结果表明,除LAMP法对耐甲氧西林葡萄球菌、流感嗜血杆菌的检测一致性极好,荧光定量PCR对耐甲氧西林葡萄球菌的检测一致性极好(Kappa≥0.75),其余病原体采用荧光定量PCR与LAMP法检测一致性均中等(0.4≤Kappa<0.75)。结论荧光定量PCR与LAMP两种检测方法对呼吸道感染病原体检出率均较高,其中LAMP法对耐甲氧西林葡萄球菌、肺炎链球菌、流感嗜血杆菌的检出率高于荧光定量PCR法。
Objective To compare the application value of fluorescence quantitative nucleic acid amplification and ring-mediated isothermal amplification in the detection of respiratory tract infection pathogens.Methods A total of 120 patients with respiratory tract infection were randomly selected from Hedong District Center for Disease Control and Prevention of Tianjin City from January 2020 to January 2023.Fluorescent quantitative nucleic acid amplification(PCR)and loop-mediated isothermal amplification(LAMP)were used to detect respiratory tract pathogens,and the detection results were compared and analyzed.Results Bacterial infection was detected by LAMP in 85 of 120 patients(positive rate was 70.83%).Bacterial infection was detected by fluorescence quantitative PCR in 72 cases(positive rate 60.00%),the difference was not statistically significant(P>0.05).Among them,the detection rate of methicillin-resistant Staphylococcus,Streptococcus pneumoniae and Haemophilus influenzae by LAMP method was higher than that by fluorescence quantitative PCR method,and the difference was statistically significant(P<0.05).Fluorescent quantitative PCR and LAMP method were used to detect 10 pathogens of respiratory tract infections.The results showed that the detection of methicillin-resistant Staphylococcus and Haemophilus influenzae by LAMP method had excellent consistency.The detection of methicillin-resistant Staphylococcus by fluorescence quantitative PCR had excellent cousistency(Kappa≥0.75).Other pathogens were detected by fluorescent quantitative PCR and LAMP with moderate consistency(0.4≤Kappa<0.75).Conclusion Both fluorescent quantitative PCR and LAMP methods have higher detection rates for respiratory infection pathogens,and the detection rate of LAMP method for methicillin-resistant Staphylococcus,Streptococcus pneumoniae and Haemophilus influenzae is higher than that of fluorescent quantitative PCR method.
作者
徐成伟
徐丽娜
XU Cheng-wei;XU Li-na(Department of Microbiology,Hedong District Center for Disease Control and Prevention,Tianjin 300000,China;不详)
出处
《中国处方药》
2024年第6期168-171,共4页
Journal of China Prescription Drug
关键词
荧光定量核酸扩增检测技术
环介导等温扩增技术
呼吸道感染
病原体
Fluorescence quantitative nucleic acid amplification detection technology
Ring-mediated isothermal amplification technique
Respiratory infection
Pathogen
