摘要
Unnatural amino acids(UAAs)have gained significant attention in protein engineering and drug development owing to their ability to introduce new chemical functionalities to proteins.In eukaryotes,genetic code expansion(GCE)enables the incorporation of UAAs and facilitates posttranscriptional modification(PTM),which is not feasible in prokaryotic systems.GCE is also a powerful tool for cell or animal imaging,the monitoring of protein interactions in target cells,drug development,and switch regulation.Therefore,there is keen interest in utilizing GCE in eukaryotic systems.This review provides an overview of the application of GCE in eukaryotic systems and discusses current challenges that need to be addressed.
基金
This work was supported by the National Key R&D Program of China(Nos.2019YFA0904200 and 2019YFA0906100)
the National Natural Science Foundation of China(No.32171464)
Shenzhen Science and Technology Innovation Program(JCYJ20180504165501371).
