摘要
甘蓝根肿病是一种由芸薹根肿菌(Plasmodiophora brassicae Woron.)侵染引发的土传病害,发生面积广,为害严重,严重威胁甘蓝产业可持续发展.为初步了解重庆市甘蓝根肿病菌生理小种的类型及其生物学特性,本研究采集重庆市部分地区甘蓝根肿病样品,采用PCR扩增法进行生理小种初步鉴定,结果显示所采集的根肿病菌菌种均以4号生理小种为主要小种.同时对该菌种的生物学特性进行了研究,结果表明,休眠孢子萌发的最适pH值为6.5,致死温度为48℃;根肿病休眠孢子的萌发率随着植株腐烂处理时间的增加而增加.在甘蓝不同生育时期接种病原菌悬液,随接种浓度的升高,根肿病发病率和病情指数整体呈上升趋势,随接种时期的推后,发病率和病情指数整体呈下降趋势,接种菌液浓度达106个/mL时病害将严重发生.病菌侵染时间越早,对甘蓝长势影响越大,而且环境中病菌浓度越高,影响越严重,接种菌液浓度达105个/mL就能对甘蓝长势造成严重影响.该研究结果为重庆市甘蓝根肿病防治提供了理论依据.
Cabbage clubroot,caused by the fungus Plasmodiophora brassicae Woron.,is a soil-borne disease.The disease is distributed in most provinces,causing serious damage and threatening the sustainable development of the cabbage industry.To preliminarily understand the types and biological characteristics of the physiological races of cabbage clubroot pathogen in Chongqing,this study collected some samples of cabbage clubroot in Chongqing,and used PCR amplification to preliminarily identify the physiological races of pathogen.The results showed that the physiological race No.4 was the main race of P.brassicaein all of collected samples.The results of study on biological characteristics of this strain indicated that:The optimum pH for the germination of dormancy spores of P.brassicae was 6.5,the lethal temperature was 48℃for the pathogen.The germination rate of spores was increased with the increase of root rot treatment time.The incidence and disease index of cabbage clubroot increased with the increase of inoculation concentration,and decreased with the postponement of inoculation time,and the disease occurred seriously when the inoculation concentration reached 106 spores/mL.The earlier of the pathogen infection time,the greater of the impact on the cabbage’s growth.The higher the concentration of pathogens in the environment,the more serious of the impact.The inoculation concentration of 105 spores/mL can have a serious impact on the growth of cabbage.The results of this study provide a theoretical basis for the prevention and control of cabbage clubroot in Chongqing.
作者
王丹
唐元满
赖婷
魏周玲
罗雪峰
况觅
李姗蓉
刘翼
杨亮
WANG Dan;TANG Yuanman;LAI Ting;WEI Zhouling;LUO Xuefeng;KUANG Mi;LI Shanrong;LIU Yi;YANG Liang(ChongqingXinong PlantProtection Technology Development Co.Ltd.,Chongqing 400715,China;Chongqing General Station of Agricultural Technology Extension,Chongqing 401121,China;Chongqing Wulong District Vegetable Industry Development Service Center,Chongqing 408500,China;College of Plant Protection,Southwest University,Chongqing 400715,China)
出处
《植物医学》
2024年第3期51-60,共10页
Plant Health and Medicine
基金
重庆市农业产业技术体系“蔬菜创新团队”项目(CQMAITS202306)。
关键词
甘蓝根肿病
生理小种
休眠孢子
生物学特性
cabbage clubroot
physiological races
dormancy spores
biological characteristics
