摘要
目的利用16S rDNA测序技术检测心房颤动患者肠道菌群的多样性及丰度变化,探究导管消融术后未复发与复发患者的肠道菌群差异并分析其相关性。方法采用前瞻性队列研究的方法,根据标准纳入2021年1月至5月我院收治的53例房颤患者作为房颤组,患者均行导管消融术,根据术后是否复发房颤将其再分为未复发组与复发组。未复发组患者47例,复发组患者6例。选取同期性别、年龄等匹配的健康体检者29例作为健康组。所有入选者均使用16S rDNA测序法检测肠道菌群。采用生物信息学方法分析房颤组与健康组、复发组与未复发组之间的肠道菌群差异。结果在alpha多样性上,房颤组患者肠道菌群多样性低于健康组。在门水平和纲水平,健康组与房颤组肠道菌群结构差异均无统计学意义(均P>0.05)。房颤组患者肠道Dinococcus-Thermophilus(Q=0.020,P=0.001)、Thermoleophilia(Q=0.023,P<0.001)、WSP-1菌纲(Q=0.025,P<0.001)、Ktedonobacteria(Q=0.028,P<0.001)、Thermomicrobia(Q=0.037,P<0.001)、Bacillales(Q=0.018,P<0.001)、Tepidisphaerales(Q=0.025,P<0.001)、JG30-KF-CM45菌目(Q=0.029,P<0.001)、JG30-KF-AS9菌目(Q=0.032,P<0.001)、DA111菌科(Q=0.039,P<0.001)、BIrii41菌科(Q=0.045,P<0.001)相对丰度均低于健康组。在属水平,房颤组和健康组菌群差异无统计学意义(P>0.05)。复发组患者肠道Proteobacteria、Spirochaetae、Betaproteobacteria、Epsilonproteobacteria、Spirochaetes、JG30_KF_AS9、Burkholderiales、Campylobacterales、Spirochaetales、Enterococcaceae、Alcaligenaceae相对丰度增高,未复发组中Neisseriales、Neisseriaceae、Christensenellaceae、Rikenellaceae相对丰度增高。两个亚组间Proteobacteria的LDA得分最高,LDA得分>4。结论房颤组患者较健康组肠道菌群多样性降低,但群落结构没有显著差异。肠道菌群失调的房颤患者进行导管消融术后较易复发,这可能是导管消融术后患者房颤复发的潜在预测因素。
Objective Using 16S rDNA sequencing technology to detect the changes in diversity and abundance of intestinal bacteria in patients with atrial fibrillation(AF),observe the differences in fecal bacteria between non-recurrence and recurrence groups after catheter ablation,and analyze their correlation.Methods In a prospective cohort study,53 patients with AF admitted from January to May 2021 were included as the AF group according to the criteria and underwent catheter ablation,then subdivided into non-recurrence group(47 cases)and recurrence group(6 cases)according to whether AF recurred after the procedure.29 healthy individuals with matching gender and age who accepted physical examination during the same period were selected as the healthy control group.All the subjects were tested for intestinal flora using the 16S rDNA assay.Results The alpha diversity of intestinal flora was lower in the AF group compared to the control group.At the phylum level and class level,there were no significant difference in community structure between the two groups(all P>0.05).The relative abundances of Dinococcus-Thermophilus(Q=0.020,P=0.001),Thermophilus(Q=0.023,P<0.001),Phylum WSP-1(Q=0.025,P<0.001),Ktedonobacteria(Q=0.028,P<0.001),Thermomicrobia(Q=0.037,P<0.001),Bacillales(Q=0.018,P<0.001),Tepidisphaerales(Q=0.025,P<0.001),Phylum JG30-KF-CM45(Q=0.029,P<0.001),Phylum JG30-KF-AS9(Q=0.032,P<0.001),Family DA111(Q=0.039,P<0.001)and Family BIrii41(Q=0.045,P<0.001)were lower in the AF group than in the control group.At the genus level,there were no significant differences between the two groups.In the AF group,the relative abundances of Proteobacteria,Spirochaetae,Betaproteobacteria,Epsilonproteobacteria,Spirochaetes,JG30_KF_AS9,Burkholderiales,Campylobacterales,Spirochaetales,Enterococcaceae and Alcaligenaceae increased in the recurrence group,and those of Neisseriales,Neisseriaceae,Christensenellaceae and Rikenellaceae increased in the non-recurrence group.In the two subgroups,the highest LDA scores were found in Proteobacteria,with LDA scores>4.Conclusion The intestinal flora diversity reduced in the AF group compared with the healthy control group,and there was no significant difference in community structure.Patients in the recurrence group had disordered gut flora compared to the non-recurrence group,which may be a potential predictor for recurrence of AF after catheter ablation.
作者
屠鸣越
陈矢
宋美玉
范梦迪
傅劲超
TU Mingyue;CHEN Shi;SONG Meiyu;FAN Mengdi;FU Jingchao(Department of General Practice,Shulan(Hangzhou)Hospital,Shulan International Medical College,Zhejiang Shuren University,Hangzhou,Zhejiang 310022,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
北大核心
2024年第5期549-557,568,共10页
Chinese Journal of Microecology
基金
浙江省公益技术研究计划项目(LGF20H020005)
浙江省医药卫生科技计划面上项目(2021442115)。
关键词
16SrDNA测序
心房颤动
导管消融术
肠道菌群
16S rDNA sequencing technology
Atrial fibrillation
Catheter ablation
Intestinal flora
