摘要
为了调查贵州省蜱虫的物种多样性及分布特征,阐明不同地理株蜱虫间的遗传进化关系,试验采用动物体表采集法与布旗法采集寄生蜱及游离蜱;使用超景深显微镜对蜱虫进行形态学鉴定。选取不同地域、种类及不能准确识别的蜱虫提取组织DNA,采用PCR法扩增蜱虫16S rDNA和COI基因片段并测序,通过BioEdit 7.0软件进行多序列比对确认蜱虫物种;使用MEGA 11.0软件对序列信息进行分析,构建基于16S rDNA和COI基因的系统进化树,对不同地理株蜱虫进行遗传进化分析。结果表明:在贵州省8个市州23个区县共采集到蜱虫2 420只,其中牛寄生蜱2 193只,羊寄生蜱92只,犬寄生蜱36只,游离蜱99只。形态学和分子生物学方法共鉴定出2种蜱虫,其中微小扇头蜱1 560只,长角血蜱860只。在系统发育进化中,微小扇头蜱、长角血蜱的16S rDNA和COI基因都分别与对应的参考基因序列聚集成一簇,贵州省大部分微小扇头蜱序列总体上聚于一个分支,只有黔东南州QDNZ1株在两个进化树中都形成了独立分支;多数长角血蜱序列也聚于同一分支,仅基于COI基因构建的系统进化树中毕节市BJS1株与其他省地理株聚在另一分支。说明贵州省家畜常见蜱种为微小扇头蜱和长角血蜱;在贵州省黔东南州存在一株微小扇头蜱特有株系,且在毕节市的长角血蜱存在省外输入的情况,这可能会增加蜱媒疾病在贵州省传播的风险。
In order to investigate the species diversity and distribution characteristics of ticks in Guizhou Province,and to elucidate the genetic evolutionary relationships among ticks of different geographical strains,in the experiment,the parasitic ticks and free ticks were collected by the animal surface collection method and Buqi method;and the ticks were identified morphologically by using super depth-of-field microscope.Stick DNA was extracted from ticks of different geographical strains,species and ticks that could not be identified accurately;PCR was used to amplify and sequence the 16S rDNA and COI gene fragments of ticks;multiple sequence comparisons were performed to confirm the species of ticks by using BioEdit 7.0 software;and the sequence information was analyzed by using MEGA 11.software,and the phylogenetic trees based on the 16S rDNA and COI genes were constructed to analyze the genetic evolution of ticks of different geographical strains.The results showed that a total of 2420 ticks were collected from 23 Districts and Counties in 8 Cities and Prefectures of Guizhou Province,including 2193 cattle-parasitized ticks,92 sheep-parasitized ticks,36 dog-parasitized ticks and 99 free ticks.Two species of ticks were identified by morphological and molecular biological methods,including Rhipicephalus microplus 1560 ticks and Haemaphysalis longicornis 860 ticks.In the phylogenetic evolution,the 16S rDNA and COI genes of Rhipicephalus microplus and Haemaphysalis longicornis were clustered with the corresponding reference gene sequences.Most of the Rhipicephalus microplus sequences in Guizhou Province were generally clustered in one branch,except for the QDNZ1 strain in Qiandongnan Prefecture,which formed independent branches in both phylogenetic trees..Most of the Haemaphysalis longicornis sequences were also clustered in the same branch,and only the BJS1 strain from Bijie City was clustered in another branch with the geographical strains from other regions in the phylogenetic tree constructed based on the COI gene.The results suggested that the common tick species in Guizhou Province were Rhipicephalus microplus and Haemaphysalis longicornis;the existence of a unique strain of Rhipicephalus microplus in Qiandongnan Prefecture,Guizhou Province,and the importation of Haemaphysalis longicornis in Bijie City from outside of the Province might increase the risk of tick-borne disease transmission in Guizhou Province.
作者
李容庭
李威仪
刘志豪
唐小敏
吴家红
LI Rongting;LI Weiyi;LIU Zhihao;TANG Xiaomin;WU Jiahong(Ministry of Education Key Laboratory of Environmental Pollution and Disease Monitoring,School of Public Health,Guizhou Medical University,Guian 550025,China;Key Laboratory of Modern Pathogenic Biology Characeristics,School of Basic Medicine,Guizhou Medical University,Guian 550025,China;Department of Parasitology,School of Basic Medicine,Guizhou Medical University,Guian 550025,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2024年第11期72-77,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2022]033-1)。
