摘要
通过高效液相色谱法对肉制品当中的酸性橙Ⅱ含量进行测量,流动相设定为甲醇-乙酸铵溶液,紫外波长调整为484 nm。检测结果表明,当浓度范围为0.5~10μg·mL^(-1)之间时,线性关系良好(r=0.9996),检出限和定量限分别为0.01 mg·kg^(-1)和0.05 mg·kg^(-1),加标回收率超过95%,标准差未超过5%,可对肉制品当中的酸性橙II含量进行快速的检测,且检测准确性较高,有较高应用价值。
The content of Acid OrangeⅡin meat products was measured by high-performance liquid chromatography,with the mobile phase set as methanol ammonium acetate solution and the UV wavelength adjusted to 484 nm.The test results showed that when the concentration range was between 0.5~10 ug·ml^(-1),the linear relationship was good(r=0.9997),and the detection limit and quantification limit were 0.01 mg·kg^(-1) and 0.05 mg·kg^(-1),respectively.The recovery rate of the added standard exceeded 95%,and the standard deviation did not exceed 5%.It can quickly detect the content of Acid OrangeⅡin meat products with high detection accuracy and high application value.
作者
刘晓楠
LIU Xiaonan(Alxa League Food and Drug Inspection Research Center,Alxa League750306,China)
出处
《现代食品》
2024年第8期163-165,共3页
Modern Food
关键词
高效液相色谱
肉制品
酸性橙Ⅱ
萃取
high performance liquid chromatography
meat products
acid OrangeⅡ
extraction
