摘要
百合(Lilium spp.)是多年生球根草本植物,包括观赏、食用和药用百合,具有较高的经济价值。百合遗传背景复杂、杂合度高、远缘杂交不亲和,且传统杂交育种周期长、育种精度低,难以快速高效地培育目标品种。目前,包括现代杂交育种、诱变育种、倍性育种、体细胞杂交育种和基因工程育种在内的现代育种技术均已在百合中应用。本文总结了百合杂交育种中远缘杂交不亲和的克服方法,诱变育种中的诱变条件,倍性育种中多倍体育种和单倍体育种主要采取的技术手段,体细胞杂交育种原生质体分离纯化和杂交的条件以及基因工程育种中百合遗传转化体系和基因编辑体系的研究进展。在此基础上,列举了不同百合育种方法和技术的研究案例,分析了不同育种技术面临的问题,并展望了百合育种技术的进一步发展和应用前景,旨在为未来百合育种技术及其应用的研究提供有价值的参考,并为更多百合新品种的创制提供依据。
Lilium spp.are perennial bulbous herbs that include ornamental,edible,and medicinal lilies all with high economic value.Since Lily is identified with complex genetic background,high heterozygosity,incompatibility in distant hybridization,long cycle of traditional cross breeding as well as low breeding accuracy,it is difficult to breed for new varieties.These modern breeding techniques,including crossbreeding,mutation breeding,polyploidization breeding,protoplast fusion breeding,and genetic engineering,have been applied in lily.This article reviews and summarizes the methods for overcoming the barriers of distant hybridization in lily hybrid breeding,the treatments in mutation breeding,the technical approaches in polyploidization breeding and haploid breeding,the methods in protoplast isolation and fusion in protoplast fusion breeding,and the advances in lily genetic transformation and editing.In addition,several examples in lily breeding methods and techniques are listed,and the problems in techniques are proposed.The future development and application prospects in lily breeding technologies are discussed.This article aims to provide insights for future research on lily breeding technologies and their applications in breeding of lily varieties.
作者
韩炘
杨柳燕
陈敏敏
李心
杨云尧
张永春
HAN Xin;YANG Liuyan;CHEN Minmin;LI Xin;YANG Yunyao;ZHANG Yongchun(Forestry and Fruit Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2024年第5期763-776,共14页
Journal of Plant Genetic Resources
基金
上海市中央引导地方科技发展资金项目(YDZX20223100003004)
上海市专业技术服务平台能力提升项目(21DZ2292300)
上海市现代农业产业技术体系项目([2023]第8-07号)。
