摘要
茶黄素是红茶的优质成分,具有良好的保健功能。本文通过高速逆流色谱(SCCC)分离技术批量提取茶黄素(TFs),选择茶黄素-3,3'-没食子酸酯(TFDG)研究其对肝癌细胞(HepG-2)、结肠腺癌细胞(Caco-2)的作用。HSCCC分离制备得到6个组分,通过高效液相(HPLC)检测,可确定组分F4中含有茶黄素(TF),F5含有茶黄素-3-没食子酸酯(TF-3-G)和茶黄素-3'-没食子酸酯(TF-3'-G),F6中含有茶黄素-3,3'-没食子酸酯(TFDG)。选择TFDG研究对癌细胞的抑制作用,CCK-8法检测其对HepG-2和Caco-2的作用,发现TFDG随着浓度的增大,时间的延长,对HepG-2和Caco-2细胞有明显的抑制作用,且呈时间和剂量依赖性。本文从分配系数K和分离因子α研究筛选出可大批量制备茶黄素的溶剂系统。有研究证实癌症的预防和醌还原酶(QR)存在某种关系,将试验数据和理论联合,采用分子对接技术验证TFDG和QR能够形成稳定的络合物,诱导QR的活性,可以增强抗癌效果,研究结果为以后深入研究茶黄素对癌细胞的作用提供参考。
Theaflavin is a high-quality component of black tea,which has good medical and health functions.High-speed countercurrent chromatography(HSCCC)has been applied for the separation of theaflavins and catechins,and examined the cytotoxic activity of the individual theaflavins against human liver cancer(HepG2)cells,Colonic adenocarcinoma(Caco-2)cells.Six components were separated and prepared by HSCCC.Further analysis of these compounds by HPLC indicated that that each component F4 contains theaflavin(TF),F5 contains theaflavin-3-gallate(TF-3-G)and theaflavin-3'-gallate(TF-3'-G),and F6 contains theaflavin-3,3'-gallate(TFDG).The results show TFDG was selected to study the inhibitory effect on cancer cells.CCK-8 was used to assess cell proliferation.TFDG has a significant inhibitory effect on HepG-2 and Caco-2 cells in a time-dependent and dose-dependent manner with the increase of concentration and time.It found a solvent system for the preparation of theaflavins in large quantities was selected based on the partitioning coefficient K and separation factor α.Also,we acknowledged that there is a certain relationship between cancer prevention and quinone reductase(QR).Combining experimental data with theory,by linking molecular docking technology was used to verify that TFDG and QR can form stable complexes,It also inducing QR activity can enhance the anticancer effect,it can providing a basis for further research on the effect of theaflavin on cancer cells.
作者
卓允允
蔡为荣
汪玉玲
王晴晴
Zhuo Yunyun;Cai Weirong;Wang Yuling;Wang Qingqing(Anhui University of Engineering,Wuhu 241000,Anhui)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2024年第6期107-116,共10页
Journal of Chinese Institute Of Food Science and Technology
基金
安徽省重点研发项目(202004a06020022)。
关键词
高速逆流色谱
肝癌细胞
结肠腺癌细胞
分子对接
high speed countercurrent chromatography
hepatoma cells
colon adenocarcinoma cells
molecular docking
