摘要
目的探讨益肾化湿颗粒对慢性肾衰竭大鼠肾组织转化生长因子-β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)表达的影响。方法60只雄性SD大鼠随机分为对照组、模型组、氯沙坦组、益肾化湿颗粒低剂量组和益肾化湿颗粒高剂量组。除对照组外,各组大鼠以腺嘌呤200 mg/(kg·d)灌胃建立慢性肾衰竭动物模型。造模成功后,对照组及模型组给予生理盐水10 ml/(kg·d)灌胃,氯沙坦组给予氯沙坦混悬液10 ml/(kg·d)灌胃,益肾化湿颗粒低剂量组和高剂量组分别给予益肾化湿颗粒混悬液2.5 g/(kg·d)、5.0 g/(kg·d)灌胃。连续给药8周后,逆转录-聚合酶链反应法(RT-PCR)和Western印迹法检测各组大鼠肾组织TGF-β1、α-SMA的mRNA及蛋白表达,Masson染色观察肾组织病理变化。结果与对照组比较,模型组TGF-β1和α-SMA的mRNA及蛋白表达显著增加(P均<0.01);与模型组比较,各治疗组TGF-β1和α-SMA的mRNA及蛋白表达均有下降(P均<0.05)。各治疗组组间比较,高剂量组、氯沙坦组TGF-β1和α-SMA的mRNA及蛋白表达低于低剂量组(P均<0.05)。Masson染色显示,各治疗组的胶原沉积均有减少。结论益肾化湿颗粒能够减轻腺嘌呤诱导慢性肾衰竭大鼠肾脏TGF-β1和α-SMA的mRNA及蛋白表达,提示其可能具有潜在的改善慢性肾衰竭大鼠肾间质纤维化的作用。
Objective To investigate the effect of Yishen Huashi granules on the expression of transforming growth factor-β1(TGF-β1)andα-smooth muscle actin(α-SMA)in the kidneys of rats with chronic renal failure.Methods Sixty male Sprague-Dawley rats were randomly divided into control group,model group,losartan group,low-dose group,and high-dose group.Except the control group,the other groups of rats were given adenine 200 mg/(kg·d)through gavage to establish the animal model of chronic renal failure.After the modeling was successful,the control group and model group were given 10 ml/(kg·d)of normal saline by gavage,while the losartan group was given 10 ml/(kg·d)of losartan suspension by gavage,and the low-dose group and high-dose group were given 2.5 g/(kg·d)and 5.0 g/(kg·d)of Yishen Huashi granules suspension by gavage,respectively.After 8 weeks of the above treatment,the mRNA and protein expression of TGF-β1 andα-SMA in renal tissues were detected by RT-PCR and Western blotting,and the pathological changes were observed after Masson staining.Results Compared with the control group,the model group showed significant increases of the mRNA and protein expression of TGF-β1 andα-SMA(P<0.01).Compared with model group,all the three treatment groups showed decreases of the mRNA and protein expression of TGF-β1 andα-SMA(P<0.05).The mRNA and protein expression of TGF-β1 andα-SMA of the high-dose group and losartan group were lower than those in the low-dose group(all P<0.05).Masson staining showed that collagen deposition was reduced in all the three treatment groups.Conclusion Yishen Huashi granules could reduce the renal mRNA and protein expression of TGF-β1 andα-SMA in rats with chronic renal failure induced by adenine,suggesting that it might have a potential effect on improving renal interstitial fibrosis in rats with chronic renal failure.
作者
王静
丁红
Wang Jing;Ding Hong(Department of Nephrology,Fourth Hospital Affiliated to Chinese Medical University,Shenyang 110034,Liaoning Province,China)
出处
《中华肾病研究电子杂志》
2024年第3期161-165,共5页
Chinese Journal of Kidney Disease Investigation（Electronic Edition）
基金
"益肾化湿颗粒"临床应用研究和基础研究开放课题(康药合字2020第384号)。
