摘要
该研究基于胶体金免疫层析技术,建立了一种快速灵敏检测奶茶中甲基苯丙胺(methamphetamine,MET)、氯胺酮(ketamine,KET)和可卡因(cocaine,COC)这3种毒品成分的方法。通过优化人工抗原包被浓度、胶体金-单克隆抗体偶联物浓度、免疫反应时间等关键因素,实现奶茶中3种毒品成分的联合检测。结果显示,该方法对MET、KET和COC标准品的检测限分别为1.60、1.59、1.39 ng/mL。该方法可用于奶茶中3种毒品成分的测定,样品平均添加回收率为90%~109%,相对标准偏差<5%。该文建立的多靶标胶体金免疫层析方法具有简单、快速、灵敏、特异等优点,为现场鉴别奶茶中非法添加的MET、KET和COC提供了新思路。
A rapid and simultaneous lateral flow immunochromatography(LFIC)method was established based on colloidal gold(CG)for testing three addictive drugs in milk tea,including methamphetamine(MET),ketamine(KET),and cocaine(COC).After optimizing the key parameters,such as the concentration of coated antigen,the combination position of the three analytes,and the immunoreaction time,the developed method could be used for the simultaneous identification of three target addictive drays in a milk tea sample with a lower concentration could be tested.The limits of detection of MET,KET,and COC were 1.60,1.59,and 1.39 ng/mL,respectively.In milk tea samples,the mean recoveries of the three types of mixed reference analytes ranged from 90%to 109%with a relative standard deviation of less than 5%.Briefly,the multiple CG-LFIC method had the advantages of easy operation,fast analysis,high sensitivity,and good specificity.It can be an effective method for simultaneous screening addictive drugs in milk tea and provides promising applications in the bio-detection field.
作者
许鹏
徐淑娟
马骉
厉佳丽
苏威
胥天然
周宇欣
徐涵悦
张明洲
XU Peng;XU Shujuan;MA Biao;LI Jiali;SU Wei;XU Tianran;ZHOU Yuxin;XU Hanyue;ZHANG Mingzhou(College of Life Sciences,China Jiliang University,Hangzhou 310018,China;Hangzhou Quickgene Sci-Tech.Co.Ltd.,Hangzhou 310002,China;Wenzhou MeiZhong Medical Laboratory,Wenzhou 325000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第14期290-296,共7页
Food and Fermentation Industries
基金
浙江省基础公益研究计划农业农村项目(LGN22C20015)
国家级大学生创新创业训练计划资助项目(202310356048)
浙江省大学生科技创新活动计划项目(2023R409A026)。
关键词
奶茶
毒品
免疫层析
胶体金
同时检测
milk tea
addictive drugs
lateral flow immunochromatography
colloidal gold
simultaneous testing
