摘要
目的观察麦粒灸对化学治疗诱导的免疫抑制小鼠Th1细胞(Th1)/Th2细胞(Th2)平衡及转录因子T盒子转录因子(T-bet)、GATA结合蛋白3(GATA3)的影响。方法32只SPF级雄性CD-1(ICR)小鼠按随机数字表法分为正常组、模型组、盐酸左旋咪唑组、麦粒灸组,每组8只。除正常组外,其他各组小鼠腹腔注射环磷酰胺(80 mg/kg,每日1次,连续3 d)制备免疫抑制小鼠模型。盐酸左旋咪唑组小鼠灌胃盐酸左旋咪唑溶液(10 mg/kg);麦粒灸组小鼠灸“关元”、双侧“足三里”、双侧“三阴交”,每穴3壮,每壮约30 s。每日1次,连续7 d。观察小鼠一般情况;检测脾脏质量及脾脏指数;HE染色法观察脾脏组织病理;蛋白质印迹法和实时荧光PCR法检测小鼠脾脏组织T-bet、GATA3、γ干扰素(IFN-γ)、白细胞介素(IL)-4蛋白及mRNA表达;酶联免疫吸附测定法检测小鼠血清IFN-γ、IL-2、IL-4含量。结果与正常组比较,模型组小鼠反应迟钝、步态不稳;脾脏质量及脾脏指数增加(均P<0.05);脾脏组织结构紊乱,脾脏组织T-bet、IFN-γmRNA及蛋白表达下降,GATA3、IL-4 mRNA及蛋白表达升高(均P<0.05);血清IFN-γ、IL-2含量减少,IL-4含量增加(均P<0.05)。与模型组比较,盐酸左旋咪唑组和麦粒灸组小鼠一般情况好转,脾脏组织结构改善,脾脏组织T-bet、IFN-γmRNA及蛋白表达下降,GATA3、IL-4 mRNA及蛋白表达升高(均P<0.05);血清IFN-γ、IL-2含量减少,IL-4含量增加(均P<0.05)。结论麦粒灸对化学治疗诱导的免疫抑制症状具有改善作用,其机制可能是调节转录因子T-bet、GATA3的表达,调节Th1/Th2平衡,从而恢复免疫平衡状态。
Objective To observe the effects of grain-sized moxibustion on Th1 cell/Th2 cell(Th1/Th2)balance and transcription factors T-box transcription factor(T-bet)and GATA binding protein 3 (GATA3)in immunosuppressive mice induced by chemotherapy.Methods According to the random number table method,32 SPF male CD-1(ICR)mice were randomly divided into the normal group,model group,levamisole hydrochloride group,and grain-sized moxibustion group,with eight mice per group.Except for the normal group,the immunosuppressive model was established by intraperitoneal injection of cyclophosphamide(80 mg/kg,once daily for three consecutive days).Mice in the levamisole hydrochloride group were given levamisole hydrochloride solution(10 mg/kg)by gavage.Mice in the grain-sized moxibustion group was given grain-sized moxibustion at"Guanyuan"(CV4),bilateral"Zusanli"(ST36),and bilateral"Sanyinjiao"(SP6),with three Zhuang at each acupoint for approximately 30 s each.The intervention was administered once daily for seven consecutive days.The general condition of mice was observed.The spleen mass and spleen index were detected.The pathological changes of spleen tissue were observed by HE staining.The protein and mRNA expressions of T-bet,GATA3,interferon-γ(IFN-γ),and interleukin(IL)-4 in spleen tissue of mice were detected by Western blotting and real-time PCR.The contents of IFN-γ,IL-2,and IL-4 in serum of mice were detected by enzyme-linked immunosorbent assay.Results Compared with the normal group,the mice in the model group were slow in response,unstable in gait;the spleen weight and spleen index were increased(P<0.05);the structure of spleen tissue was disordered,the mRNA and protein expressions of T-bet and IFN-γin spleen tissue were decreased,and the mRNA and protein expressions of GATA3 and IL-4 were increased(P<0.05);the contents of IFN-γand IL-2 in serum were decreased,and the content of IL-4 was increased(P<0.05).Compared with the model group,the general condition of mice in the levamisole hydrochloride group and the grain-sized moxibustion group was improved,the structure of spleen tissue was improved,the mRNA and protein expressions of T-bet and IFN-γin spleen tissue were decreased,and the mRNA and protein expressions of GATA3 and IL-4 were increased(P<0.05);the contents of IFN-γand IL-2 in serum were decreased,and the content of IL-4 was increased(P<0.05).Conclusion Grain-sized moxibustion can significantly improve the immunosuppressive symptoms induced by chemotherapy.The mechanism may be through regulating the expressions of transcription factors T-bet and GATA3,regulating Th1/Th2 balance,and thus restoring the immune balance.
作者
朱涛
王祯芝
任佳
程艳婷
高珍
冀雨芳
苗晋玲
冀来喜
ZHU Tao;WANG Zhenzhi;REN Jia;CHENG Yanting;GAO Zhen;JI Yufang;MIAO Jinling;JI Laixi(College of Acupuncture-Moxibustion and Tuina,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;The Second Clinical Medicine College,Shanxi University of Chinese Medicine,Jinzhong 030619,China;Fourth Department of Acupuncture and Moxibustion,Shanxi Acupuncture-Moxibustion Hospital,Taiyuan 030006,China)
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2024年第6期818-825,共8页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金项目(No.82074549)
山西省中医药管理局科研项目(No.2022ZYYZ075,No.2023ZYYA105)
山西中医药大学2023年度科技创新能力培育计划项目(No.2023PY-YS-15,No.2023PY-YS-04)。
