摘要
目的:本研究旨在通过网络药理学探讨“黄芪-丹参”治疗冠状动脉粥样硬化性心脏病的有效成分、作用靶点以及相关途径。方法:检索“黄芪-丹参”两味中药主要成分和作用靶点,采用中药系统药理学数据库与分析平台(TCMSP)筛选出“黄芪-丹参”的活性成分及靶点,利用人类基因数据库GeneGards和DrugBank检索冠状动脉粥样硬化性心脏病相关靶点,取药物与疾病的共同靶点绘制韦恩图。以STRING在线分析平台分析药物与疾病共同靶点,进行蛋白质-蛋白质相互作用网络构建。利用Metascape数据库对药物与疾病共同靶点进行基因本体论(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析,并利用Cytocsape 3.9.1软件构件“中药-有效成分-作用靶点-疾病-通路”网络图。结果:共筛选出“黄芪-丹参”85个有效化学成分,包括山柰酚、槲皮素、丹参酮IIA等。100个作用靶点与冠状动脉粥样硬化性心脏病有关。GO分析结果:分子功能672条,生物过程4753条,细胞组分672条。KEGG结果显示有184条信号通路发挥对冠状动脉粥样硬化性心脏病的治疗作用。结论:本研究探讨“黄芪-丹参”治疗冠状动脉粥样硬化性心脏病可能通过多成分、多靶点、多通路,为后续研究探讨提供新的理论依据。
Objective:This study was aimed to investigate the effective components,targets and related pathways of Huangqi(Radix Astragali)-Danshen(Radix et Rhizoma Salviae Miltiorrhizae)in the treatment of coronary atherosclerotic heart disease through network pharmacology.Methods:The main components and action targets of Huangqi-Danshen were searched.Traditional Chinese medicine systems pharmacology database and analysis platform was adopted to screen the active ingredients and targets of Huangqi-Danshen.Human gene databases GeneGards and DrugBank were used to search CHD related targets.The common target of drugs and diseases were selected to draw Venn diagram.The common targets of drugs and diseases were analyzed by STRING online analysis platform,and the protein-protein interaction network was constructed.The Metascape database was used for gene ontology(GO)functional enrichment analysis of common drug and disease targets,and the Kyoto encyclopedia of genes and genomes genomes(KEGG)pathway enrichment analysis was conducted.The Cytocsape 3.9.1 software was used to construct the TCM-effective component-target-disease-pathway network diagram.Results:A total of 85 effective chemical components were selected from Huangqi-Danshen,including kaempferol,quercetin,tanshinone IIA,etc.,and 100 targets were associated with coronary heart disease.The results of GO analysis showed 672 biological functions,4753 biological processes and 672 cytological components.KEGG results showed that 184 signaling pathways were obtained to play a role in the treatment of coronary heart disease.Conclusion:This study explored the possibility of Huangqi-Danshen participating in the treatment of coronary heart disease through multi-component,multi-target and multi-pathway,providing a new theoretical basis for subsequent research.
出处
《中医临床研究》
2024年第17期32-41,共10页
Clinical Journal Of Chinese Medicine
关键词
冠状动脉粥样硬化性心脏病
黄芪-丹参药对
益气活血
网络药理学
信号通路
Coronary atherosclerotic heart disease
Huangqi-Danshen
Benefiting Qi and activating blood circulation
Network pharmacology
Signal pathway
