摘要
目的:提高人丹的质量标准。方法:采用薄层鉴别、显微鉴别及HPLC、GC含量测定法对人丹11味药材进行质量标准提高研究。结果:建立了人丹中肉桂、草豆蔻显微鉴别方法,甘草、桔梗薄层鉴别方法,以上鉴别方法专属性、重复性强。建立了同时测定人丹中甘草苷与甘草酸HPLC含量测定方法,甘草苷与甘草酸分别在浓度5.154~103.1μg/mL、25.51~510.3μg/mL范围内呈较好线性关系,平均加样回收率分别为106.8%、102.1%。建立了同时测定人丹中樟脑、薄荷脑、冰片(合成龙脑)GC含量测定方法,其分别在13.09~242.2μg/mL、55.34~608.7μg/mL、14.46~159.0μg/mL范围内呈较好线性关系,加样回收率分别为102.7%、103.6%、105.1%。结论:提高后的质量标准可更准确地进行定性、定量检测,能够很好地用于人丹的质量控制。
Objective:To improve the quality standard of Rendan.Methods:11 kinds of herbs in Rendan were identified by TLC,microscopic identification,GC identification,and HPLC.Results:Cinnamomum and herba amomum,and glycyrrhiza and platycodon,were respectively identified by microscopical identification and TLC with high specificity and repeatability.HPLC method for the determination of glycyrrhizin and glycyrrhizic acid was established.Glycyrrhizin and glycyrrhizic acid respectively presented a good linear relationship under the concentration of 5.154-103.1μg/mL,and 25.51-510.3μg/mL,and the recovery rate was average of 107.0%,and 102.1%.Furthermore,the GC method for determining camphora,menthols,and bornol simultaneously was established,showing good linear relationship under the concentration of 13.09-242.2μg/mL,55.34-608.7μg/mL,and 14.46-159.0μg/mL,respectively,with an average recovery rate of 102.7%,103.6%,and 105.1%.Conclusion:The improved quality standard can be used for qualitative and quantitative detection more accurately,and can be used for quality control.
作者
伍柏坚
符皖
罗燕玉
彭绍忠
许伟滨
黄晓丹
Wu Bojian;Fu Wan;Luo Yanyu;Peng Shaozhong;Xu Weibin;Huang Xiaodan(Guangzhou Wanglaoji Pharmaceutical Company Limited,Guangzhou 510450,China;Guangdong Provincial Key Laboratory of Framous Chinese Medicine and Herbal Tea,Guangzhou 510450,China)
出处
《亚太传统医药》
2024年第7期99-103,共5页
Asia-Pacific Traditional Medicine
