摘要
目的 探讨纤维介素2(FGL2)在上皮性卵巢癌中的表达情况及其对人卵巢癌SKOV3细胞生物学特性的影响及相关机制。方法 (1)取2018年7月—2020年5月在河北省人民医院行手术治疗的40例浆液性卵巢癌以及16例卵巢浆液性囊腺瘤患者的切除组织标本,免疫组化法检测组织标本中FGL2蛋白表达情况。(2)取SKOV3细胞,实验设空白组、FGL2 siRNA转染组和阴性对照siRNA组,采用Western blot法检测细胞中FGL2蛋白表达情况,以确定FGL2 siRNA转染效率;后续通过CCK-8法以及Transwell实验检测FGL2 siRNA转染组和阴性对照siRNA组SKOV3细胞增殖、侵袭及迁移能力,Western blot法检测细胞中白细胞介素-6(IL-6)、信号转导和转录激活因子3(STAT3)蛋白表达情况,观察沉默FGL2对SKOV3细胞增殖、侵袭及迁移能力和IL-6/STAT3信号通路的影响。结果 浆液性卵巢癌患者组织标本中FGL2蛋白高表达率明显高于卵巢浆液性囊腺瘤患者[80.0%(32/40)比37.5%(6/16),P<0.05]。FGL2 siRNA转染组FGL2蛋白相对表达量明显低于阴性对照siRNA组(P<0.05);FGL2 siRNA转染组转染后48 h、72 h、96 h、120 h的SKOV3细胞增殖活性均明显低于同期阴性对照siRNA组(P均<0.05);Transwell加基质胶侵袭实验和不加基质胶迁移实验显示FGL2 siRNA转染组穿膜细胞数均明显少于阴性对照siRNA组(P均<0.05);FGL2 siRNA组IL-6和p-STAT3蛋白相对表达量均明显低于阴性对照siRNA组(P均<0.05)。结论 卵巢癌组织中存在FGL2高表达,沉默FGL2抑制IL-6/STAT3信号通路的活性可影响卵巢癌细胞的生物学特性,推测FGL2可作为卵巢癌诊治的靶点之一。
Objective It is to explore the expression of fibronectin 2(FGL2)in epithelial ovarian cancer,and to study its effect on the biological characteristics of human ovarian cancer SKOV3 cells and correlated mechanisms.Methods①The resected tissue specimens were taken from 40 cases of patients with serous ovarian carcinoma and 16 cases of patients with serous cystadenoma of ovary who underwent surgical treatment in Hebei General Hospital,and the expression of FGL2 protein in the tissue specimens was detected by immunohistochemistry.②The SKOV3 cells were taken and divided into FGL2 siRNA transfection group and negative control siRNA group,the FGL2 protein expression in the cells was detected by Western blot to determine the efficiency of FGL2 siRNA transfection,then the proliferation,invasion and migration ability of SKOV3 cells in the FGL2 siRNA transfection group and negative control siRNA group were subsequently detected by CCK-8 assay and Transwell assay,and the protein expressions of interleukin-6(IL-6),signal transducer and activator of transcription 3(STAT3)in the cells were detected by Western blot assay,so as to observe the effect of silencing of FGL2 on the proliferation,invasion and migration ability of SKOV3 cells and on IL-6/STAT3 signaling pathway.Results The high expression rate of FGL2 protein in tissue specimens from patients with serous ovarian carcinoma was significantly higher than that from patients with serous cystadenoma of ovary[80.0%(32/40)versus 37.5%(6/16),P<0.05].The relative expression of FGL2 protein in the FGL2 siRNA-transfection group was significantly lower than that of the negative control siRNA group(P<0.05);the proliferative activity of SKOV3 cells at 48 h,72 h,96 h and 120 h after transfection in the FGL2 siRNA-transfection group was significantly lower than that of the negative control siRNA group(P<0.05);Transwell invasion assay with matrix gel and migration assay without matrix gel showed that the number of membrane-penetrating cells in the FGL2 siRNA transfection group was significantly less than that in the negative control siRNA group(P<0.05);the relative expression of IL-6 and p-STAT3 proteins in the FGL2 siRNA group were both significantly lower than those in the negative control siRNA group(both P<0.05).Conclusion There is high FGL2 expression in ovarian cancer tissues,silencing FGL2 to inhibit the activity of IL-6/STAT3 signaling pathway can inflence the biological characteristics of ovarian epithelial cancer cells,which suggests that FGL2 may serve as one of the potential targets for the diagnosis and treatment of ovarian cancer.
作者
王新玲
李楠
赵武杰
王斌
王雅卓
范静
李娜
WANG Xinling;LI Nan;ZHAO Wujie;WANG Bin;WANG Yazhuo;FAN Jing;LI Na(Hebei General Hospital,Shijiazhuang 050051,Hebei,China;Hebei Medical University,Shijiazhuang 050011,Hebei,China)
出处
《现代中西医结合杂志》
CAS
2024年第12期1642-1647,1665,共7页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
河北省卫健委重点科技研究计划项目(20170292)。
