摘要
为探究花旗松素与葛根素的协同抗氧化能力,将两者分别按照体积比1:1、1:2和2:1比例进行复配,通过DPPH、ABTS和FRAP法测定其体外抗氧化活性,进一步运用SynergyFinder软件进行HSA模型评价协同效果。结果表明,花旗松素和葛根素均具有一定的抗氧化能力,当花旗松素6.25~25μg/mL与葛根素2~25 mg/mL,花旗松素0~6.25μg/mL与葛根素3.12~12.5μg/mL,花旗松素2.5~10μg/mL与葛根素0~250μg/mL三个浓度范围内1:1复配时,分别对DPPH自由基、ABTS阳离子自由基和FRAP的协同抗氧化能力最强,HSA模型评分最高,分别为13.486、8.383和1.930。因此,选用花旗松素与葛根素进行复配使用,在体积比1:1具有较强的协同抗氧化能力。
To investigate the synergistic antioxidant activity of taxifolin and puerarin,the synergistic antioxidant activity of the two compounds with 1:1,1:2,and 2:1(volume ratio)was assessed using DPPH,ABTS,and FRAP assays and the HSA model was utilized to evaluate the possible synergistic effects by SynergyFinder software.Results revealed that both taxifolin and puerarin possessed antioxidant properties.When the ratio of taxifolin(6.25-25μg/mL)to puerarin(2-25 mg/mL),taxifolin(0-6.25μg/mL)to taxifolin(3.12-12.5μg/mL),taxifolin(2.5-10μg/mL)to puerarin(0-250μg/mL)were 1:1,the HSA scores for DPPH free radicals,ABTS cationic radicals,and FRAP were the highest,with 13.486,8.383,and 1.930,respectively.Thus,it could be concluded that taxifolin and puerarin with a ratio of 1:1 exhibited a synergistic antioxidant capacity.
作者
王云富
崔彩芳
孙兆岳
徐奔
陈富春
沈维军
万发春
程安玮
WANG Yunfu;CUI Caifang;SUN Zhaoyue;XU Ben;CHEN Fuchun;SHEN Weijun;WAN Fachun;CHENG Anwei(College of Food Science and Technology,Engineering Center of Rapeseed Oil Nutrition Health and Deep Development of Hunan Province,Hunan Agricultural University,Changsha 410128,China;College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第15期132-141,共10页
Food and Fermentation Industries
基金
国家重点研发计划项目(2022YFD1301101-1)
湖南省科技创新团队项目(2021RC4060)。
