摘要
花生荚果膨大过程直接决定了荚果大小,深入研究该过程有助于明确荚果大小的调控机制。本研究以不同荚果大小的花生品种(系)为研究对象,分析荚果发育早期转录水平的变化,并利用加权基因共表达网络分析(WGCNA)确定与荚果发育相关的关键基因。结果表明,差异基因主要富集到跨膜受体蛋白激酶活性、UDP−葡萄糖基转移酶活性、植物细胞壁、膜的锚固成分和细胞壁的生物合成功能上;MAPK信号通路−植物、植物激素信号转导、植物−病原菌互作、苯丙烷生物合成以及淀粉和蔗糖代谢是荚果发育早期主要富集到的代谢通路。WGCNA共鉴定到29个模块,其中Antiquewhite4和Darkolivegreen模块与荚果长、宽呈极显著相关,对两个关键模块内权重值最高的200个基因对进行核心基因分析,发现gene-LOC112797233、gene-LOC112743864、gene-LOC112701973、gene-LOC112764826和gene-LOC112710700等多个潜在的候选基因在荚果发育过程中起重要作用。此外,对模块内基因的GO富集分析表明,Antiquewhite4和Darkolivegreen模块可能分别与次生细胞壁的形成及细胞周期的调控相关。研究结果有助于进一步了解花生荚果发育的分子机制。
Peanut pod expansion process directly determines the pod size,and in-depth study of this process is helpful to clarify the regulation mechanism of pod size.In this study,peanut varieties with different pod sizes were used to analyze the changes of transcription level in the early stage of pod development,weighted gene co-expression network analysis(WGCNA)was used to identify the key genes related to pod development.The results showed that DEGs of different materials were significantly enriched in transmembrane receptor protein kinase activity,UDP-glucosyltransferase activity,plant-type cell wall,anchored component of membrane and cell wall biogenesis;MAPK signaling pathway-plant,plant hormone signal transduction,plant-pathogen interaction,phenylpropanoid biosynthesis and starch and sucrose metabolism were the main significant enrichment pathways.A total of 29 modules were identified by WGCNA,of which Antiquewhite4 and Darkolivegreen modules were significantly correlated with pod length and width.Hub gene analysis of 200 gene pairs with the highest weights in two key modules showed that gene-LOC112797233,gene-LOC112743864,gene-LOC112701973,gene-LOC112764826 and gene-LOC112710700 were found to play an important role in pod development.GO enrichment analysis of genes in key modules showed that Antiquewhite4 and Darkolivegreen modules may be related to the formation of secondary cell wall and the regulation of cell cycle,respectively.The results will be helpful for further understanding the molecular mechanism of peanut pod development.
作者
吕正昊
史晓龙
周东英
任婧瑶
钟超
刘喜波
张鹤
康树立
赵新华
于海秋
王传堂
LYU Zheng-hao;SHI Xiao-long;ZHOU Dong-ying;REN Jing-yao;ZHONG Chao;LIU Xi-bo;ZHANG He;KANG Shu-li;ZHAO Xin-hua;YU Hai-qiu;WANG Chuan-tang(College of Agronomy,Shenyang Agricultural University,Shenyang 110161,China;Shandong Peanut Research Institute,Qingdao 266000,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2024年第4期808-818,共11页
Chinese Journal of Oil Crop Sciences
基金
国家现代农业产业技术体系(CARS-13)
沈阳市科学技术计划(21-110-3-17)
辽宁省“揭榜挂帅”科技攻关专项(2021JH/10400034)。
关键词
花生
荚果
发育
转录组
WGCNA
核心基因
peanut
pod
development
transcriptome
weighted gene co-expression network(WGCNA)
hub gene
