摘要
试验旨在探究由葛根素(Puerarin,PR)和茯苓多糖(Poria cocos polysaccharide,PCP)组成的复合物(PR+PCP,PP)对猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)感染仔猪结肠的保护作用。采用单因素试验设计,通过使用PEDV感染仔猪肠道损伤模型进行试验,选用30头体重相近的7日龄健康仔猪,随机分成三个处理组:对照组、PEDV组和PP+PEDV组,每组10个重复,每个重复1头猪。试验期为14 d,其中预试期为4 d,正试期为10 d,试验期间各组饲喂相同的日粮,正试期内每晚给PP+PEDV组仔猪口腔灌服PP,将PR(0.5 mg/kg BW),PCP(20 mg/kg BW)混合溶于人工奶中;其余两组灌服相同体积人工奶。第10天向PEDV组和PP+PEDV组的仔猪口腔灌服PEDV(毒价为105.5 TCID50,用PBS溶液稀释),对照组仔猪灌服相同体积的PBS溶液。结果表明:①与对照组相比,PEDV组仔猪血清中总胆固醇(TC)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)含量显著降低(P<0.05);三酯甘油(TG)、总胆红素(TB)、直接胆红素(DB)和尿素氮(BUN)的含量显著升高(P<0.05);结肠隐窝深度(Crypt depth,CD)显著升高(P<0.05);结肠中总超氧化物歧化酶(T-SOD),髓过氧化物酶(MPO)活性显著升高(P<0.05);结肠中干扰素刺激基因15(ISG15)相对表达量显著升高(P<0.05);结肠白细胞介素(IL)-1β、白细胞介素-6(IL-6)、C-X-C基序趋化因子配体2(CXCL2)和再生胰岛衍生蛋白3γ(REG3G)的相对表达量显著升高(P<0.05)。②与PEDV组相比,PP+PEDV组仔猪血清中TG、谷草转氨酶(AST)和BUN的水平显著降低(P<0.05);结肠CD显著降低(P<0.05);结肠中ISG15基因相对表达量显著降低(P<0.05);结肠中IL-1β、IL-6、CXCL2和REG3G基因相对表达量显著降低(P<0.05)。综上所述,在试验条件下灌服PP能够缓解PEDV感染仔猪血清生化指标异常情况,并通过改善PEDV感染仔猪结肠的隐窝深度,缓解结肠炎性反应及影响机体免疫反应来缓解PEDV感染造成的仔猪结肠肠道损伤。
The aim of this study was to investigate the protective effect of the complex of puerarin and poria cocos polysaccharide(PR+PCP,PP)on the colon of piglets infected with porcine epidemic diarrhea virus(PEDV).A single factor experimental design was used in this study.Thirty healthy 7-day-old piglets with similar body weight were randomly divided into three treatment groups(10 replicates per treatment,1 piglet per replicate):control group,PEDV group and PP+PEDV group.The test period was 14 days,including 4 days of prefeeding and 10 days of trialperiod.During the trialperiod,all groups were fed the same diet.During the trialperiod,PP+PEDV group piglets were given PP by orala dministration every night,and PR(0.5 mg/kg BW)and PCP(20 mg/kg BW)were mixed and dissolved inartificial milk;The other two groups were fed with the same volume of artificial milk.On the 10th day,the pigletsin the PEDV group and PP+PEDV group were orally administered with PEDV(105.5 TCID50,diluted with PBS solution),and the piglets in the control group wereorally administered with the same volume of PBS solution.The results were as follows:①compared with the control group,the contents of TC,HDL and LDL in serum of piglets in PEDV group were significantly decreased(P<0.05);the contents of TB,DB and BUN were significantly increased(P<0.05);the crypt depth of colon significantly increased(P<0.05);the activity of T-SOD in the colon was significantly decreased(P<0.05),the activity of MPO was significantly increased(P<0.05);the relative expression level of ISG15 in colon was significantly increased(P<0.05);the relative expression levels of IL-1β,IL-6,CXCL2 and REG3G in colon were significantly increased(P<0.05).②Compared with PEDV group,the contents of TG and BUN and the activity of AST in serum of piglets in PP+PEDV group were significantly decreased(P<0.05);the crypt depth of the colon was significantly decreased(P<0.05);and the relative expression level of ISG15 in colon was significantly decreased(P<0.05);the relative expression levels of IL-1β,IL-6,CXCL2 and REG3G in colon were significantly decreased(P<0.05).In conclusion,under the conditions of this experiment,PP administration can alleviate the abnormal serum biochemical indexes in PEDV-infected piglets.Furthermore,PP administration alleviates the colon damage in piglets caused by PEDV infection by improving the crypt depth of colon and colonic inflammatory response.
作者
谌梦
胡锦超
田晓榕
赵迪
王蕾
吴涛
张焱焱
侯永清
CHEN Meng;HU Jinchao;TIAN Xiaorong;ZHAO Di;WANG Lei;WU Tao;ZHANG Yanyan;HOU Yongqing(Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Hubei Wuhan 430023,China;Hubei Collaborative Innovation Center of Animal Nutrition and Feed Safety,Wuhan Polytechnic University,Hubei Wu-han 430023,China)
出处
《饲料工业》
CAS
北大核心
2024年第16期47-54,共8页
Feed Industry
基金
国家自然科学基金面上项目[32172763]
武汉轻工大学科研项目资助[2024J04]。
关键词
仔猪
结肠
葛根素
茯苓多糖
猪流行性腹泻病毒
免疫反应
piglet
colon
puerarin
poria cocos polysaccharide
porcine epidemic diarrhea virus
immune response
