摘要
目的了解2019—2022年包头市市售肉及肉制品中弯曲菌(Campylobacter)带菌率,并对其分离菌株的抗生素敏感性、耐药基因及分子分型等病原学特征进行研究。方法2019—2022年共采集市售肉及肉制品300份,分离培养获得弯曲菌,测定其抗生素敏感性、耐药基因及基因突变位点,并通过脉冲场凝胶电泳(PFGE)对菌株进行分子分型。结果300份肉及肉制品中共检出弯曲菌142株,其中生禽肉中检出率为65.71%(138/210),结肠弯曲菌检出率显著高于空肠弯曲菌(χ^(2)=12.48,P<0.01);仅有3株弯曲菌对全部11种抗生素敏感,有26株细菌耐受全部11种抗生素并有130株发生多重抗生素耐药,主要耐药性表现在喹诺酮(萘啶酸、环丙沙星)及四环素类药物;在喹诺酮耐药弯曲菌中均检测到gyr A基因发生C-243-T的位点突变,并在91.09%的四环素耐药弯曲菌中检测到tet O耐药基因;142株弯曲菌分为105个PFGE型别,相似度在35.1%~100%,无明显优势型别条带。结论包头市肉及肉制品中存在弯曲菌污染,且在生禽肉中污染尤为严重;弯曲菌对喹诺酮及四环素类抗生素耐药程度高且多重耐药情况较为严重,PFGE型别呈多态性。
Objective To understand the contamination status of Campylobacter in meat products of Baotou,the pathogenic characteristics such as drug resistance genes,antibiotic sensitivities,molecular typing were analyzed.Methods Three hundred meat products were collected from 2019 to 2022,which were cultured for Campylobacter isolation.Sensitivities to antibiotics,drug resistance genes and mutation sites of isolated Campylobacter were detected and genetic characteristics were analyzed by pulsed field gel electrophoresis(PFGE)method as well.Results A total of 142 Campylobacter were isolated from 300 meat products,of which the isolation rate of raw poultry was 65.71%(138/210).The detection rate of Campylobacter coli was significantly higher than that of Campylobacter jejuni(χ^(2)=12.48,P<0.01).Only 3 strains were sensitive to all 11 antibiotics,and drug resistance were mainly manifested in quinolones(naphthalenic acid,ciprofloxacin)and tetracycline.26 strains were resistant to all 11 antibiotics and 130 strains were resistant to multiple antibiotics.Mutations in C-257-T site of gyrA gene had occyred in quinolone-resistant campylobacter.And tetO gene was detected in 91.09%of tetracycline-resistant strains.All Campylobacter could be divided into 105 PFGE types.The similarity coefficients of different PFGE types ranged from 35.1%to 100%and there was no a dominant PFGE type.Conclusion Campylobacter pollution in meat products in Baotou have been detected,and the pollution is particularly serious in raw poultry products.Campylobacter has a high degree of resistance to quinolones and tetracycline antibiotics,and the PFGE types are polymorphic.
作者
庞梦婷
彭景贤
薄力
高蒙
刘欣
陈凯旋
PANG Mengting;PENG Jingxian;BO Li;GAO Meng;LIU Xin;CHEN Kaixuan(Baotou Center for Disease Control and Prevention,Inner Mongolia Baotou 014030,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2024年第5期588-594,共7页
Chinese Journal of Food Hygiene
基金
包头市青年创新人才项目(2021102530)
包头市卫生健康科技计划(wsjkkj063)。
关键词
肉及肉制品
弯曲菌
耐药基因
耐药性
脉冲场凝胶电泳
食源性致病菌
Meat and meat products
Campylobacter
drug resistance genes
drug resistance
pulsed field gel electrophoresis
foodborne bacterial pathogens
