摘要
采用QuEChERS-超高效液相色谱串联质谱法建立一种测定鱼肉中13种糖皮质激素的分析方法。结果表明,13种糖皮质激素在1~100 ng·mL^(-1)时线性关系良好,相关系数均大于0.99,定量限为0.2~5.0 μg·kg^(-1),加标回收率在75.1%~95.7%。该方法前处理简单快速,回收率和精密度较高,可用于鱼肉中13种糖皮质激素的检测。
A method to determine 13 glucocorticoids in fish using QuEChERS combined with ultra-high performance liquid chromatography-tandem mass spectrometry was developed.By optimizing pre-processing methods and conducting methodological validation,the results indicated that the 13 glucocorticoids exhibited good linearity within the range of 1 ng·mL~(-1) to 100 ng·mL~(-1),with correlation coefficients all greater than 0.99,the limits of quantification ranged from 0.2 μg·kg~(-1) to 5.0 μg·kg~(-1),and the recovery rates were between 75.1% and 95.7%.This method has simple and fast pre-processing,high recovery rate and precision,and can be used for the detection of 13 glucocorticoids in fish meat.
作者
仵军红
李琦
赵世红
朱盈蕊
樊亚光
王留留
WU Junhong;LI Qi;ZHAO Shihong;ZHU Yingrui;FAN Yaguang;WANG Liuliu(Zhengzhou Product Quality Inspection Center,Zhengzhou 450000,China;Henan Institute of Food and Salt Industry Inspection Technology,Zhengzhou 450000,China)
出处
《食品安全导刊》
2024年第20期76-79,共4页
China Food Safety Magazine
基金
河南省市场监督管理局科技计划项目(2022sj130,2022sj129)。
